Name: IL-12 (p70) Antibody, anti-human, REAfinity™
Availability: InStock

Intracellular flow cytometry

Conjugate:	APC
Recommended dilution:	1:10
Remark:	The antibody is suited for staining of formaldehyde-fixed cells.
Tested:	QC tested
Products used:	130-103-738, 130-103-673

Mass cytometry

Conjugate:	pure
Products used:	130-108-051

Specifications for IL-12 (p70) Antibody, anti-human, REAfinity™

Overview

Interleukin-12 (IL-12) is a 70–75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is produced mainly by monocytes and macrophages and by dendritic cells, primarily in response to stimulation with bacterial or viral antigens. IL-12 has also been described to be produced by non-hematopoietic cells such as keratinocytes and epidermoid carcinomas. IL-12 displays various effects on both NK and T cells, playing an essential role in the interaction between innate and adaptive immunity. It induces the production of IFN-γ alone or in synergy with IL-2, acts as a co-stimulator of both NK and T cell proliferation, and enhances cytolytic activity of both cell types. Moreover, IL-12 promotes the development of T helper 1 (Tʜ1)-specific immune responses by promoting the differentiation of CD4

⁺

cells towards a Tʜ1 phenotype while inhibiting the production of Tʜ2 cells. Clone REA123 reacts with the biologically active form of human IL-12 p70 heterodimer, but not with the p40 monomer or homodimer.

Additional information: Clone REA123 displays negligible binding to Fc receptors.

Alternative names

IL12A, CLMF, NFSK, P35, IL12B, IMD28, IMD29, NKSF

Detailed product information

Technical specifications

Clone	REA123
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	IL-12 (p70)
Alternative names of antigen	IL12A, CLMF, NFSK, P35, IL12B, IMD28, IMD29, NKSF
Molecular mass of antigen [kDa]	57(sum of molecular weights of subunits)
Distribution of antigen	macrophages, monocytes, dendritic cells
Entrez Gene ID	3592, 3593
RRID	AB_2652337, AB_2652338, AB_2652339, AB_2652340, AB_2652341

References for IL-12 (p70) Antibody, anti-human, REAfinity™

Publications

D´Andrea, A. et al. (1992) Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J. Exp. Med. 176: 1387-1398
Gubler, U. et al. (1991) Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc. Natl. Acad. Sci. U.S.A. 88(10): 4143-4147
Trinchieri, G. et al. (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat. Rev. Immunol. 3(2): 133-146

Applications

Specifications

Specifications for IL-12 (p70) Antibody, anti-human, REAfinity™

Overview

⁺

Additional information: Clone REA123 displays negligible binding to Fc receptors.

Alternative names

IL12A, CLMF, NFSK, P35, IL12B, IMD28, IMD29, NKSF

Detailed product information

Technical specifications

Clone	REA123
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	IL-12 (p70)
Alternative names of antigen	IL12A, CLMF, NFSK, P35, IL12B, IMD28, IMD29, NKSF
Molecular mass of antigen [kDa]	57(sum of molecular weights of subunits)
Distribution of antigen	macrophages, monocytes, dendritic cells
Entrez Gene ID	3592, 3593
RRID	AB_2652337, AB_2652338, AB_2652339, AB_2652340, AB_2652341

References

References for IL-12 (p70) Antibody, anti-human, REAfinity™

Publications

D´Andrea, A. et al. (1992) Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J. Exp. Med. 176: 1387-1398
Gubler, U. et al. (1991) Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc. Natl. Acad. Sci. U.S.A. 88(10): 4143-4147
Trinchieri, G. et al. (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat. Rev. Immunol. 3(2): 133-146

IL-12 (p70) Antibody, anti-human, REAfinity™

IL-12 (p70) Antibody, anti-human, REAfinity™

Intracellular flow cytometry

Mass cytometry

Specifications for IL-12 (p70) Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References for IL-12 (p70) Antibody, anti-human, REAfinity™

Publications

Applications

Specifications

Specifications for IL-12 (p70) Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References

References for IL-12 (p70) Antibody, anti-human, REAfinity™

Publications

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IL-12 (p70) Antibody, anti-human, REAfinity™

IL-12 (p70) Antibody, anti-human, REAfinity™

Intracellular flow cytometry

Mass cytometry

Specifications for IL-12 (p70) Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References for IL-12 (p70) Antibody, anti-human, REAfinity™

Publications

Applications

Specifications

Specifications for IL-12 (p70) Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References

References for IL-12 (p70) Antibody, anti-human, REAfinity™

Publications

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