The IgM
+
Memory B Cell Isolation Kit has been developed for the isolation of IgM
+
memory B cells from human PBMCs.

Data and images for
IgM
+
Memory B Cell Isolation Kit
, human

Figures

Figure 1

IgM
+
memory B cells were isolated from human PBMCs using the IgM
+
Memory B Cell Isolation Kit, an LD Column and a MidiMACS™ Separator, an MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-IgM-APC and CD27-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
PBMCs before separation
Pre-enriched memory B cells
View details

Figure 1

IgM
+
memory B cells were isolated from human PBMCs using the IgM
+
Memory B Cell Isolation Kit, an LD Column and a MidiMACS™ Separator, an MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-IgM-APC and CD27-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

IgM
+
memory B cells were isolated from human PBMCs using the IgM
+
Memory B Cell Isolation Kit, an LD Column and a MidiMACS™ Separator, an MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-IgM-APC and CD27-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Isolated IgM
+
memory B cells
View details

Figure 1

IgM
+
memory B cells were isolated from human PBMCs using the IgM
+
Memory B Cell Isolation Kit, an LD Column and a MidiMACS™ Separator, an MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-IgM-APC and CD27-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
IgM
+
Memory B Cell Isolation Kit
, human

Overview

The IgM
+
Memory B Cell Isolation Kit has been developed for the isolation of IgM
+
memory B cells from human PBMCs.

Detailed product information

Background information

IgM
+
memory B cells, defined as quiescent antigen-experienced B cells, are generated in response to T cell–dependent and T cell–independent antigens. They are able to react quickly to a recurrent antigenic challenge thereby providing serological immune protection.

Detailed separation procedure

IgM
+
memory B cells are isolated by depletion of unwanted cells and subsequent positive selection with Anti-IgM MicroBeads. Non-B cells (T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (Glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

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IgM
+
Memory B Cell Isolation Kit
, human

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