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C57BL/6 mouse splenocytes were stained with CD314 (NKG2D) antibodies as well as with Anti-NK1.1 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (control: left images). Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
Clone | CX5 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG1κ |
Isotype control | Isotype Control Antibody, rat IgG1 |
Host | rat |
Type of antibody | Primary antibodies |
Species | mouse |
Antigen | CD314 (NKG2D) |
Alternative names of antigen | Klrk1, NKG2-D, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member, Nkg2d |
Molecular mass of antigen [kDa] | 27 |
Distribution of antigen | T cells, NK cells, macrophages, dendritic cells, NKT cells |
Entrez Gene ID | 27007 |
RRID | AB_2657376, AB_2657377, AB_2657378, AB_2657379, AB_2657380, AB_2657381, AB_2657382, AB_2657375 |
Clone | CX5 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG1κ |
Isotype control | Isotype Control Antibody, rat IgG1 |
Host | rat |
Type of antibody | Primary antibodies |
Species | mouse |
Antigen | CD314 (NKG2D) |
Alternative names of antigen | Klrk1, NKG2-D, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member, Nkg2d |
Molecular mass of antigen [kDa] | 27 |
Distribution of antigen | T cells, NK cells, macrophages, dendritic cells, NKT cells |
Entrez Gene ID | 27007 |
RRID | AB_2657376, AB_2657377, AB_2657378, AB_2657379, AB_2657380, AB_2657381, AB_2657382, AB_2657375 |
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