Name: CD314 (NKG2D) Antibody, anti-mouse
Availability: InStock

Flow cytometry

Conjugate:	PE
Recommended dilution:	1:10
Remark:	Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Tested:	QC tested
Products used:	130-103-367, 130-102-728

MICS (MACSima Imaging Cyclic Staining)

Conjugate:	APC
Recommended dilution:	1:10
Fixation:	PFA
Tested:	during development
Products used:	130-103-368, 130-102-729
Compatible applications:	IF, IHC

Specifications for CD314 (NKG2D) Antibody, anti-mouse

Overview

Clone CX5 recognizes mouse CD314 (NKG2D), a type II transmembrane homodimeric glycoprotein belonging to the C-type lectin-like receptor family. CD314 in mice is expressed on activated CD8

⁺

αβ T cells, NK cells, subsets of γδ T cells, IFN-producing killer dendritic cells, subsets of NKT cells, and activated macrophages. For signaling, CD314 associates with signaling adapters DAP10 and DAP12 and transduces signal via the PI3-kinase pathway and the Syk-Zap70 pathways, respectively. CD314 recognizes multiple ligands, which are either uregulated in response to cellular stresses such as heat shock, infection, and DNA damage or are constitutively expressed, such as on tumor cells. Known ligands of CD314 in mice include murine UL16-binding protein-like transcript (MULTI), H60 family proteins and the five retinoic acid-inducible proteins RAE-1α, β, γ, δ, ε. Interaction of CD314 with its ligands regulates cytotoxic response towards the cells bearing the cognate ligands.

Alternative names

Klrk1, NKG2-D, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member, Nkg2d

Detailed product information

Technical specifications

Clone	CX5
Clonality	monoclonal
Isotype	rat IgG1κ
Isotype control	Isotype Control Antibody, rat IgG1
Host	rat
Type of antibody	Primary antibodies
Species	mouse
Antigen	CD314 (NKG2D)
Alternative names of antigen	Klrk1, NKG2-D, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member, Nkg2d
Molecular mass of antigen [kDa]	27
Distribution of antigen	T cells, NK cells, macrophages, dendritic cells, NKT cells
Entrez Gene ID	27007
RRID	AB_2657376, AB_2657377, AB_2657378, AB_2657379, AB_2657380, AB_2657381, AB_2657382, AB_2657375

References for CD314 (NKG2D) Antibody, anti-mouse

Publications

Takada, A. et al. (2008) Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D. J. Immunol. 180: 1678-1685
McMahon, C. et al. (2002) The role of the NKG2D immunoreceptor in immune cell activation and natural killing. Immunity 17(1): 19-29
Diefenbach, A. et al. (2000) Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages. Nat. Immunol. 1(2): 119-126
Yokoyama, W. M. et al. (2002) NKG2D recruits two distinct adapters to trigger NK cell activation and costimulation. Nat. Immunol. 3(12): 1150-1155

Applications

Specifications

Specifications for CD314 (NKG2D) Antibody, anti-mouse

Overview

Clone CX5 recognizes mouse CD314 (NKG2D), a type II transmembrane homodimeric glycoprotein belonging to the C-type lectin-like receptor family. CD314 in mice is expressed on activated CD8

⁺

Alternative names

Klrk1, NKG2-D, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member, Nkg2d

Detailed product information

Technical specifications

Clone	CX5
Clonality	monoclonal
Isotype	rat IgG1κ
Isotype control	Isotype Control Antibody, rat IgG1
Host	rat
Type of antibody	Primary antibodies
Species	mouse
Antigen	CD314 (NKG2D)
Alternative names of antigen	Klrk1, NKG2-D, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member, Nkg2d
Molecular mass of antigen [kDa]	27
Distribution of antigen	T cells, NK cells, macrophages, dendritic cells, NKT cells
Entrez Gene ID	27007
RRID	AB_2657376, AB_2657377, AB_2657378, AB_2657379, AB_2657380, AB_2657381, AB_2657382, AB_2657375

References

References for CD314 (NKG2D) Antibody, anti-mouse

Publications

Takada, A. et al. (2008) Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D. J. Immunol. 180: 1678-1685
McMahon, C. et al. (2002) The role of the NKG2D immunoreceptor in immune cell activation and natural killing. Immunity 17(1): 19-29
Diefenbach, A. et al. (2000) Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages. Nat. Immunol. 1(2): 119-126
Yokoyama, W. M. et al. (2002) NKG2D recruits two distinct adapters to trigger NK cell activation and costimulation. Nat. Immunol. 3(12): 1150-1155

CD314 (NKG2D) Antibody, anti-mouse

CD314 (NKG2D) Antibody, anti-mouse

Flow cytometry

MICS (MACSima Imaging Cyclic Staining)

Specifications for CD314 (NKG2D) Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References for CD314 (NKG2D) Antibody, anti-mouse

Publications

Applications

Specifications

Specifications for CD314 (NKG2D) Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References

References for CD314 (NKG2D) Antibody, anti-mouse

Publications

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CD314 (NKG2D) Antibody, anti-mouse

CD314 (NKG2D) Antibody, anti-mouse

Flow cytometry

MICS (MACSima Imaging Cyclic Staining)

Specifications for CD314 (NKG2D) Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References for CD314 (NKG2D) Antibody, anti-mouse

Publications

Applications

Specifications

Specifications for CD314 (NKG2D) Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References

References for CD314 (NKG2D) Antibody, anti-mouse

Publications

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CD314 (NKG2D) Antibody, anti-mouse