Clone:
REA959
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
LFA-2, Ly-37

Extended validation for CD2 Antibody, anti-mouse, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD2. Mouse splenocytes were stained with CD2antibodies and with a suitable counterstaining. As a control, CD2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD2. Mouse splenocytes were stained with CD2antibodies and with a suitable counterstaining. As a control, CD2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD2. Mouse splenocytes were stained with CD2antibodies and with a suitable counterstaining. As a control, CD2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD2. Mouse splenocytes were stained with CD2antibodies and with a suitable counterstaining. As a control, CD2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD2. Mouse splenocytes were stained with CD2antibodies and with a suitable counterstaining. As a control, CD2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD2 (REA959). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD2 (REA959). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD2 (REA959). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD2 Antibody, anti-mouse, REAfinity™

Overview

Clone REA959 recognizes the type I transmembrane glycoprotein CD2, also known as LFA-2 or Ly-37. In mouse, CD2 is expressed on all lymphocytes except a subpopulation of intraepithelial T lymphocytes. It is a major receptor for CD48 and is involved in activation and differentiation of T cells. CD2-mediated cell-cell adhesion can be blocked by REA959.
Additional information: Clone REA959 displays negligible binding to Fc receptors.

Alternative names

LFA-2, Ly-37

Detailed product information

Technical specifications

CloneREA959
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD2
Alternative names of antigenLFA-2, Ly-37
Molecular mass of antigen [kDa]36
Distribution of antigenB cells, T cells
Entrez Gene ID12481
RRIDAB_2727243, AB_2727286, AB_2727244, AB_2727287, AB_2727245, AB_2727290, AB_2727248, AB_2727288, AB_2727246, AB_2727289, AB_2727247, AB_2727284, AB_2727242, AB_2727285

Resources for CD2 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD2 Antibody, anti-mouse, REAfinity™

Publications

  1. Yagita, H. et al. (1989) Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells. Proc. Natl. Acad. Sci. U.S.A. 86: 645-649
  2. Nakamura, T. et al. (1990) Relative contribution of CD2 and LFA-1 to murine T and natural killer cell functions. J. Immunol. 145: 3628-3634
  3. Davis, S. J. and van der Merwe, P. A. (1996) The structure and ligand interactions of CD2: implications for T-cell function. Immunol. Today 17(4): 177-187

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