CD11c MicroBeads UltraPure, mouse

Name: CD11c MicroBeads UltraPure, mouse
Availability: InStock

CD11c MicroBeads UltraPure, mouse

In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Product data and images for CD11c MicroBeads UltraPure, mouse

Figures

Figure 1

CD11c

⁺

DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

CD11c

⁺

dendritic cells

Figure 1

CD11c

⁺

DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

CD11c

⁺

DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD11c MicroBeads UltraPure, mouse

Overview

In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Detailed product information

Background information

The mouse CD11c antigen is present in dendritic cells in lymphoid organs and blood, in Langerhans cells in the epidermis, in dendritic cell progenitors in the bone marrow, and in in vitro generated bone marrow–derived dendritic cells. In mouse spleen and lymph node, CD11c is expressed at high levels on conventional CD11c

⁺

CD45R

^–

mPDCA-1

^–

dendritic cells, and at moderate levels on CD11c

⁺

CD45R

⁺

mPDCA-1

⁺

plasmacytoid dendritic cells. CD11c is reported to be weakly expressed on NK cells, B cells, and T cell subsets. About 1–3% of splenocytes, 2% of bone marrow cells, as well as <1% of lymph node cells and thymocytes express CD11c.

Downstream applications

DCs isolated with CD11c MicroBeads have been used for phenotypical and functional analyses, e.g. T cell activation, polarization, and tolerance induction in different experimental murine models. CD11c MicroBeads were used to isolate highly pure DCs from spleen and lymph nodes

^1,2

, Peyer's patches

³

, colonic lamina propria

⁴

, bone marrow

⁵

, epidermis

⁶

, lung

⁷

, liver

⁸

, or brain

⁹

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD11c MicroBeads UltraPure, mouse

Documents and Protocols

Brochures/posters

The broadest portfolio for your dendritic cell research

Brochures/posters

All you need for myeloid cell research - Quick guide

Brochures/posters

MACS® Cell Pre-Enrichment for flow sorting and analysis

Brochures/posters

Human and mouse dendritic cell generation

Scientific posters

Simple and fast method for isolation of mouse cross-presenting dendritic cells

References

Myeloid cells in cancer research - selected references

References for CD11c MicroBeads UltraPure, mouse

Publications

Williamson, E. et al. (2002) Regulation of mucosal dendritic cell function by receptor activator of NF-κB (Rank)/Rank ligand interactions: impact on tolerance induction. J. Immunol. 169: 3606-3612
Ing, R. et al. (2006) Interaction of mouse dendritic cells and malaria-infected erythrocytes: uptake, maturation, and antigen presentation. J. Immunol. 176: 441-450
Kadaoui. K. A. and Corthésy, B. et al. (2004) Isolation of dendritic cells from mouse Peyer’s patches using magnetic cell sorting. MACS&more 8(1): 10-12
Krajina, T. et al. (2003)
Colonic lamina propria dendritic cells in mice with CD4
⁺
T cell-induced colitis.
Eur. J. Immunol. 33: 1073-1083
Hou, W. S. and van Parijs, L. et al. (2004) A Bcl-2-dependent molecular timer regulates the lifespan and immunogenicity of dendritic cells. Nat. Immunol. 5: 583-589
Sato, T. et al. (2002) Consequences of OX40-OX40 ligand interactions in langerhans cell function: enhanced contact hypersensitivity responses in OX40L-transgenic mice. Eur. J. Immunol. 32: 3326-3335
Swanson, K. A. et al. (2004) Flt3-Ligand, Il-4, GM-CSF, and adherencemediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function. J. Immunol. 173: 4875-4881
Johansson, C. and Wick, M. J. (2004)
Liver dendritic cells present bacterial antigens and produce cytokines upon
Salmonella
encounter.
J. Immunol. 172: 2496-2503
Fischer, H. G. et al. (2000)
Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with
Toxoplasma gondii
.
J. Immunol. 164: 4826-4834

Data and images

Data and images for CD11c MicroBeads UltraPure, mouse

Figures

Figure 1

CD11c

⁺

DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

CD11c

⁺

dendritic cells

Figure 1

CD11c

⁺

DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

CD11c

⁺

DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for CD11c MicroBeads UltraPure, mouse

Overview

In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Detailed product information

Background information

The mouse CD11c antigen is present in dendritic cells in lymphoid organs and blood, in Langerhans cells in the epidermis, in dendritic cell progenitors in the bone marrow, and in in vitro generated bone marrow–derived dendritic cells. In mouse spleen and lymph node, CD11c is expressed at high levels on conventional CD11c

⁺

CD45R

^–

mPDCA-1

^–

dendritic cells, and at moderate levels on CD11c

⁺

CD45R

⁺

mPDCA-1

⁺

plasmacytoid dendritic cells. CD11c is reported to be weakly expressed on NK cells, B cells, and T cell subsets. About 1–3% of splenocytes, 2% of bone marrow cells, as well as <1% of lymph node cells and thymocytes express CD11c.

Downstream applications

DCs isolated with CD11c MicroBeads have been used for phenotypical and functional analyses, e.g. T cell activation, polarization, and tolerance induction in different experimental murine models. CD11c MicroBeads were used to isolate highly pure DCs from spleen and lymph nodes

^1,2

, Peyer's patches

³

, colonic lamina propria

⁴

, bone marrow

⁵

, epidermis

⁶

, lung

⁷

, liver

⁸

, or brain

⁹

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Resources

Resources for CD11c MicroBeads UltraPure, mouse

Documents and Protocols

Brochures/posters

References for CD11c MicroBeads UltraPure, mouse

Publications

Williamson, E. et al. (2002) Regulation of mucosal dendritic cell function by receptor activator of NF-κB (Rank)/Rank ligand interactions: impact on tolerance induction. J. Immunol. 169: 3606-3612
Ing, R. et al. (2006) Interaction of mouse dendritic cells and malaria-infected erythrocytes: uptake, maturation, and antigen presentation. J. Immunol. 176: 441-450
Kadaoui. K. A. and Corthésy, B. et al. (2004) Isolation of dendritic cells from mouse Peyer’s patches using magnetic cell sorting. MACS&more 8(1): 10-12
Krajina, T. et al. (2003)
Colonic lamina propria dendritic cells in mice with CD4
⁺
T cell-induced colitis.
Eur. J. Immunol. 33: 1073-1083
Hou, W. S. and van Parijs, L. et al. (2004) A Bcl-2-dependent molecular timer regulates the lifespan and immunogenicity of dendritic cells. Nat. Immunol. 5: 583-589
Sato, T. et al. (2002) Consequences of OX40-OX40 ligand interactions in langerhans cell function: enhanced contact hypersensitivity responses in OX40L-transgenic mice. Eur. J. Immunol. 32: 3326-3335
Swanson, K. A. et al. (2004) Flt3-Ligand, Il-4, GM-CSF, and adherencemediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function. J. Immunol. 173: 4875-4881
Johansson, C. and Wick, M. J. (2004)
Liver dendritic cells present bacterial antigens and produce cytokines upon
Salmonella
encounter.
J. Immunol. 172: 2496-2503
Fischer, H. G. et al. (2000)
Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with
Toxoplasma gondii
.
J. Immunol. 164: 4826-4834

Video

How to isolate CD11c+ dendritic cells from mouse spleen

Watch how easy it is to manually separate DCs from mouse tissue using MACS Technology.

Related products for
CD11c MicroBeads UltraPure, mouse

6 products available | view all view less

Seems like you are coming from USA!
Do you want to visit our website in your country?

CD11c MicroBeads UltraPure, mouse

CD11c MicroBeads UltraPure, mouse

Product data and images for CD11c MicroBeads UltraPure, mouse

Figures

Figure 1