Coat a 24-well culture dish with 0.01 % Poly-L-lysine overnight at 37 °C. Wash three times with ddH₂O the following day.
Prepare the following cell culture medium: MACS Neuro Medium containing 2 % MACS NeuroBrew®-21, 1 % penicillin/streptomycin and 0.5 mM L-glutamine, 10 ng/mL Human PDGF-AA, and 10 ng/mL Human FGF-2.
Dissociate mouse neonatal brain using the Neural Tissue Dissociation Kit (P) or Neural Tissue Dissociation Kit (T). Follow the protocol of the kit data sheet.
Download data sheet
Isolate O4-positive oligodendrocytes using the Anti-O4 MicroBeads, human, mouse, rat. Follow the protocol of the kit data sheet.
Download data sheet
Notes:
Column | Max. number of labeled cells | Max. number of total cells | Separator |
---|---|---|---|
Positive selection | |||
MS | 1×107 | 2×107 | MiniMACS™, OctoMACS™ |
LS | 2×107 | 4×107 | MidiMACS™, QuadroMACS™ |
autoMACS | 5×107 | 1×108 | autoMACS Pro |
Multi-24 | 2×107 | 4×107 | MultiMACS Cell24 |
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