Miltenyi Biotec-tested panel 28 (MBTP 28)
This application protocol describes the flow cytometric analysis of virus-specific T cells after enrichment of IFN-γ+ cells from human blood. IFN-γ+ cells were enriched using the CliniMACS® Cytokine Capture System (IFN-gamma) to obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|7-AAD Staining Solution||-||Viability||-||655–730 (blue)|
|CD4||REA623||T helper cells||VioGreen™||525/50 (violet)|
|CD3||REA613||T cells||FITC||525/50 (blue)|
|CD8||REA734||Cytotoxic T cells||APC-Vio® 770||750 LP (red)|
|IFN-γ||45-15||Activation marker||PE||585/40 (blue)|
|CD45RO||REA611||Memory T cells||PE-Vio 770||750 LP (blue)|
|CD62L||145/15||Naive and memory T cells||APC||655–730 (red)|
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
If not otherwise specified, all marks displayed on the Miltenyi Biotec Internet site and documents are subject to the trademark rights of Miltenyi Biotec, including each of Miltenyi Biotec's primary brands, its model name plates, and its corporate logos and emblems.
For copyright note and a complete list of trademarks visit www.miltenyibiotec.com/legalnotes.
Seems like you are coming from USA!
Do you want to visit our website in your country?