The CD4
+
T Cell Isolation Kit, mouse was developed for the untouched isolation of CD4
+
T cells from single-cell suspensions of mouse spleen and/or lymph nodes.
This kit uses the new fast mouse protocol and replaces the CD4
+
T Cell Isolation Kit II (# 130-095-248), allowing isolation of CD4
+
T cells in just 18 minutes.

Data and images for
CD4
+
T Cell Isolation Kit
, mouse

Figures

Figure 1

A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS Dissociator. CD4
+
T cells were isolated from this single-cell suspension using the CD4
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD4 T Cell Cocktail (# 130-092-916) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
CD4
+
T cells after depletion of non-CD4
+
T cells
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS Dissociator. CD4
+
T cells were isolated from this single-cell suspension using the CD4
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD4 T Cell Cocktail (# 130-092-916) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS Dissociator. CD4
+
T cells were isolated from this single-cell suspension using the CD4
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD4 T Cell Cocktail (# 130-092-916) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
CD4
+
T Cell Isolation Kit
, mouse

Overview

The CD4
+
T Cell Isolation Kit, mouse was developed for the untouched isolation of CD4
+
T cells from single-cell suspensions of mouse spleen and/or lymph nodes.
This kit uses the new fast mouse protocol and replaces the CD4
+
T Cell Isolation Kit II (# 130-095-248), allowing isolation of CD4
+
T cells in just 18 minutes.

Detailed product information

Detailed separation procedure

CD4
+
T cells are isolated by depletion of non CD4
+
T cells using a cocktail of biotin-conjugated antibodies against CD8a, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, Ter-119 and TCRγ/δ as primary labeling reagent. The cells are then magnetically labeled with Anti-Biotin MicroBeads. The magnetically labeled non-target cells are depleted by retaining them on a MACS
®
Column in the magnetic field of a MACS Separator, while the unlabeled target cells pass through the column.

Downstream applications

The CD4
+
T Cell Isolation Kit, mouse is used where untouched CD4
+
T cells are desired or where further magnetic separation into T cell subsets is required.

Columns

LS or autoMACS
®
Columns.

Resources for
CD4
+
T Cell Isolation Kit
, mouse

Reviews for
CD4
+
T Cell Isolation Kit
, mouse

CD4+ T Cell Isolation Kit for Mouse

  • 1
  • 2
  • 3
  • 4
  • 5

CD4+ T Cell Isolation Kit, mouse (130-104-454)

We are interested in the CD4-specific population after we harvest splenocytes from mice. We use this kit all the time when we want a good population. The populations are always very clean.

Total CD4+ T Isolation Kit

  • 1
  • 2
  • 3
  • 4
  • 5

CD4+ T Cell Isolation Kit, mouse (130-104-454)

For general T helper cell isolation, the yields from this are quite good. Useful for both lymphoid and non-lymphoid tissues.

Clean Isolation of CD4+ T Cells from Splenocytes

  • 1
  • 2
  • 3
  • 4
  • 5

CD4+ T Cell Isolation Kit, mouse (130-104-454)

This kit has served our needs well for isolation CD4+ T Cells from mouse splenocytes.

Related products for
CD4
+
T Cell Isolation Kit
, mouse

4 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?