Interleukin 7 (IL-7) is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells.
MACS GMP Recombinant Human IL-7 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human IL-7

Figures

Figure 1

View details
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-7. The peak corresponds to the calculated molecular mass of 17382 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-7. The peak corresponds to the calculated molecular mass of 17382 Da.

Figure 2

View details
Human IL-7 biological activity.
Activity of Human IL-7, GMP grade (blue bar) was compared to another commercially available product (black bar).

Figure 2

Human IL-7 biological activity.
Activity of Human IL-7, GMP grade (blue bar) was compared to another commercially available product (black bar).

Figure 3

View details
MACS GMP Recombinant Human IL-7 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP IL-7.

Figure 3

MACS GMP Recombinant Human IL-7 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP IL-7.

Specifications for
MACS
®
GMP Recombinant Human IL-7

Overview

Interleukin 7 (IL-7) is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells.
MACS GMP Recombinant Human IL-7 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

The primary sources of IL-7 are non-hematopoietic stromal cells in bone marrow, thymus, and lymphoid organs and tissues. IL-7 is a member of the type I cytokine family. It is related to IL-2 and signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain.

Applications

MACS GMP Recombinant Human IL-7 can be used for a variety of applications, including the
ex vivo
generation of antigen-specific cytotoxic T lymphocytes.
2,3

Biological activity

  • The specific activity is determined by proliferation assay according to Ishihara
    et al.1
    using mouse 2E8 cells. The proliferation assay was calibrated with the reference standard for human IL-7 (NIBSC code 90/530) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 5×
    10
    7
    U/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human IL-7

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human IL-7

Publications

  1. Ishihara, K. et al. (1991) Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B-to B-cell transition. Dev. Immunol. 1: 149-161
  2. Zhang, H. et al. (2007) 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. J. Immunol. 179: 4910-4918
  3. Kaneko et al. (2009) IL-7 and IL-15 allow the generation of suicide gene-modified alloreactive self-renewing central memory human T lymphocytes. Blood 113: 1006-1015

Related products for
MACS
®
GMP Recombinant Human IL-7

1 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?