Miltenyi Biotec-tested panel 17 (MBTP 17)
This application protocol describes the flow cytometric analysis of dendritic cells (DCs) after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD11b||REA592||cDC1/cDC2 discrimination||VioBlue®||450/50 (violet)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|CD172a (SIRPα)||P84*||cDC1/cDC2 discrimination||Alexa 488||525/50 (blue)|
|XCR1||REA707||cDC1/cDC2 discrimination||PE||585/40 (blue)|
|MHC Class II||REA813||Conventional dendritic cells||PE-Vio® 615||655-730 (blue)|
|CD317 (PDCA-1)||REA818||Plasmacytoid dendritic cells||PE-Vio 770||750LP (blue)|
|Siglec-H||REA819||Plasmacytoid dendritic cells||APC||655-730 (red)|
|CD11c||REA754||Conventional dendritic cells||APC-Vio 770||750LP (red)|
* The recombinant antibody recognizing the same epitope is CD172a (SIRPα) Antibody, anti-mouse, FITC, REAfinity™ (# 130-123-157).
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