Vio Bright V423 is Miltenyi Biotec’s brightest dye excited at 405 nm. In addition to high brightness this dye shows a significantly improved signal-to-noise ratio, minimal intra-laser compensation and a superior detection of rare antigens.
Vio Bright V423 is a synthetic dye which is 5-times brighter compared to the VioBlue® Dye. This makes Vio Bright V423 an excellent choice for multiparameter cell analysis.
Overview of fluorophore characteristics
|Excitation max||399 nm|
|Emission max||420 nm|
|Common filter set (FC)||450/50 nm|
|Stain index||5 out of 5|
|Photostablility||Photostable to ambient light|
Compared to polymer-based fluorochromes with high fluorescence intensities, such as Brilliant Violet 421 and Super Bright 436, Vio Bright V423 exhibits a similar or brighter degree of fluorescence. Increased brightness of Vio Bright V423 conjugates is observed across different antigens; the data below show examples of CD19 and CD56 staining.
Compared to the other dyes excited by violet lasers, Vio Bright V423 shows minimal spillover into the neighboring detection channels. Thus, with a minimal change in compensation settings, Vio Bright V423 provides the benefit of a brighter dye.
Compensation matrix for a CD19 antibody conjugated to different fluorochromes excited by a violet laser. Values depict the percentage of compensation required for the respective detection filter.
|Conjugate||V1-A (450/50nm)||V2-A (525/50 nm)||V3-A (579/34 nm)||V4-A (615/20 nm)||V5-A (667/30 nm)|
|V1-A (450/50 nm)||CD19-Vio Bright V423||100||4||1|
|V2-A (525/50 nm)||CD19-BV510||40||100||60||40||6|
|V3-A (579/34 nm)||CD19-BV570||40||3||100||65||10|
|V5-A (667/30 nm)||CD19-BV650||30||3||52||100|
Miltenyi Biotec–tested panels (MBTPs) are a collection of multicolor panels that are optimized and pre-designed for specific flow cytometry applications. They are developed and validated by Miltenyi Biotec’s R&D experts, as well as by the customers who actually use Miltenyi Biotec antibodies.