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Mouse macrophages are extensively studied in tissues and tumor. For reliable isolation of mouse macrophages, gentle dissociation of lymphoid tissue is key. Mouse spleen can be dissociated into single-cell suspensions using our Spleen Dissociation Kit, mouse in combination with the gentleMACS™ Dissociator with Heaters. Mechanical dissociation with enzymatic degradation of the extracellular matrix maintains the structural integrity of tissues.
We have developed a fast and efficient way to isolate mouse macrophages based on their specific F4/80 markers by using Anti-F4/80 MicroBeads UltraPure, mouse.
Isolation of F4/80+ macrophages. F4/80+ cells were isolated from spleen single-cell suspension using Anti-F4/80 MicroBeads UltraPure, two MS Columns, and an OctoMACS™ Separator. The cells were fluorescently stained with CD45-VioBlue®, CD11b-APC, and Anti-F4/80-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.
We offer various reagents and kits for the isolation of mouse myeloid cells from tissues and tumors. Download the poster and find a comprehensive overview of all myeloid cell markers and products for convenient isolation and analysis.
Working with MDSCs? The Myeloid-Derived Suppressor Cell Isolation Kit, mouse has been developed for the isolation of CD11b+Gr-1+ myeloid cells from mouse lymphoid tissue.
Macrophages can be generated in vitro from human monocytes using Human M-CSF, premium grade or Human GM-CSF, premium grade .