Diagnostic research of multiple myeloma*
 

Application notes and a scientific poster for diagnostic research of multiple myeloma

• Automated Isolation of CD138⁺ cells (application note)
• Saving valuable hands-on time – automated CD138+ plasma cell enrichment (application note)
• A standardized and reproducible method for expansion and activation of human primary B cells (scientific poster)

Plasma cell isolation 
 

Cell isolation solutions for cytogenetic analysis and efficient enrichment of CD138⁺ plasma cells is a prerequisite for valid cytogenetic analysis of bone marrow samples. Conventional methods for the isolation of CD138⁺ plasma cells requires laborious density gradient centrifugation and generation of mononuclear cells prior to any plasma cell purification. MACSprep™ Multiple Myeloma CD138 MicroBeads, human allow the isolation of CD138⁺ plasma cells directly from bone marrow or peripheral blood without the need for gradient centrifugation or erythrocyte lysis. The minimal manipulation of sample ensures high integrity of CD138⁺ plasma cells, suitable for further cellular analysis (flow cytometry), cytogenetic analysis (FISH), or molecular analysis (sequencing, PCR, microarray).

Find more information on MACSprep products.

Cytogenetic and flow analysis of malignant plasma cells
 

Most multiple myeloma studies perform fluorescent in situ hybridization (FISH) analysis with isolated CD138⁺ plasma cells. More studies are incorporating new molecular methods to diagnose multiple myeloma, such as next generation sequencing (NGS) and single nucleotide polymorphism (SNP) array. These assays require DNA isolation from CD138⁺ plasma cells, as well as analysis of phenotype of malignant plasma cells, which can be determined by flow cytometry.
 

FISH is a standard method for identifying genomic aberrations in multiple myeloma. It can also be performed at the interphase of cells, iFISH. In multiple myeloma applications, iFISH is primarily performed. To analyze the cells at interphase directly, isolation of CD138⁺ cells is necessary to show the aberrant chromosome/genes are from plasma cells 
 

References:

A. Palumbo et al.; J Clin Oncol; Revised International Staging System for Multiple Myeloma: A Report From International Myeloma Working Group 

P. Martin et al,; Modern Pathology, hMLH1 and MGMT inactivation as a mechanism of tumorigenesis in monoclonal gammopathies
 

To gain more information on the isolated malignant plasma cells flow analysis can be performed. Our flow reagents ensure that the data you generate on your flow cytometer is reliable and consistent:

Build your own B cell flow panel with our  Flow Panel Builder
 

Gating strategy to define malignant plasma cells by flow cytometry
For this application we have designed a specific panel that can help you with the analysis of malignant plasma cells.