Find the products and resources you are looking for!
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Increasing number of studies focus on the distinct functions of classical an non-classical monocyte subsets. We have developed specific kits for the separation of monocytes and monocyte subsets.
Using our new StraightFrom™ Portfolio we provide tools for fast and easy isolation of monocytes directly from whole blood, buffy coat, and leukapheresis without the need for density gradient centrifugation, wash steps, erythrocyte lysis, and cell counting. Using StraightFrom™ Buffy Coat CD14 MicroBead Kit, human you can get pure CD14+ monocytes directly from buffy coat in less than 30 minutes.
Isolation of CD14+ monocytes from buffy coat. Separation of a buffy coat sample using the StraightFrom™ Buffy Coat CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD15-APC, CD14-PE, as well as CD45‑VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Large numbers of DCs for basic research and immunotherapies can be generated in vitro from monocytes using several differentiation/maturation protocols. To obtain functional Mo-DCs that activate T cells efficiently, reliable procedures using high-quality reagents are necessary.