Clone:
REA179
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
TRA, IMD7, TCRA, TCRD, TRAC, TCRAV7S1, TRAV7

Extended validation for TCR Vα7.2 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA179
3C10++
Cells were incubated with an excess of purified unconjugated TCR Vα7.2 (REA179) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for TCR Vα7.2. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vα7.2 antibodies and with a suitable counterstaining. As a control, TCR Vα7.2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vα7.2. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vα7.2 antibodies and with a suitable counterstaining. As a control, TCR Vα7.2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vα7.2. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vα7.2 antibodies and with a suitable counterstaining. As a control, TCR Vα7.2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for TCR Vα7.2. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vα7.2 antibodies and with a suitable counterstaining. As a control, TCR Vα7.2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vα7.2 (REA179). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vα7.2 (REA179). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCR Vα7.2 (REA179). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vα7.2 Antibody, anti-human, REAfinity™

Overview

Clone REA179 recognizes the TCR chain Vα7.2, which together with Jα33 segment forms the invariant T cell receptor. Human Vα7.2-Jα33 invariant T cells are preferentially localized in the gut and are often termed as mucosal-associated T (MAIT) cells. MAIT cells are restricted to MHC class I like molecules, such as MR1. Further, MAIT cells are suggested to be involved in regulation of immune response at the mucosal surfaces, which are the primary sites for pathogen entry.
Additional information: Clone REA179 displays negligible binding to Fc receptors.

Alternative names

TRA, IMD7, TCRA, TCRD, TRAC, TCRAV7S1, TRAV7

Detailed product information

Technical specifications

CloneREA179
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTCR Vα7.2
Alternative names of antigenTRA, IMD7, TCRA, TCRD, TRAC, TCRAV7S1, TRAV7
Distribution of antigenT cells
Entrez Gene ID28686
RRIDAB_2811544, AB_2802031, AB_2802015, AB_2653666, AB_2653667, AB_2653668, AB_2653669, AB_2653670, AB_2653671, AB_2653674, AB_2653675, AB_2653676, AB_2653677, AB_2811576

Resources for TCR Vα7.2 Antibody, anti-human, REAfinity™

Certificates

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References for TCR Vα7.2 Antibody, anti-human, REAfinity™

Publications

  1. Treiner, E. et al. (2003) Selection of evolutionarily conserved mucosal-associated invariant T cells by MR1. Nature 422(6928): 164-169
  2. Wang, Y. et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J. Exp. Med. 213(11): 2413-2435
  3. Qualai, J. et al. (2016) Expression of CD11c is associated with unconventional activated T cell subsets with high migratory potential. PLoS One 11(4): e0154253
  4. Gold, M. C. and Lewinsohn, D. M. (2013) Co-dependents: MR1-restricted MAIT cells and their antimicrobial function. Nat. Rev. Microbiol. 11(1): 14-19

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