Fluidics
| Pipetting volume range | 0.02–50 mL |
|---|---|
| Uptake flow rate | 0.25–8 mL/min |
| Liquids | autoMACS Running Buffer – MACS Separation Buffer autoMACS Washing Solution Storage solution (70% Ethanol) Bleach solution (1% Sodium hypochlorite solution) |
Technical specifications
| Working temperature | 15–30 °C |
|---|---|
| Storage temperature | Room temperature |
| Humidity | 20% to 80% relative humidity, noncondensing |
| Altitude max. | 2000 m |
| Emission sound pressure level at workstation | <70 dB(A) |
| USB ports | 2 x USB 2.0 ports (rear panel) 2 x USB 3.0 ports (display) |
| Additional ports | RS232 Interface (labeled COM) Ethernet Interface (labeled LAN) CAN Bus + DC Output (labeled Power CAN) Bottle Sensor Interface (labeled Bottle Sensor) For service only: CAN-Bus + DC-Output (labeled CAN) |
| RAM | 4 GB |
| Mass storage | SSD, 64 GB |
| Input voltage | 100–240 V~, 50/60 Hz |
| Maximum power consumption | 200 W |
| Average power consumption | 50 W |
| Fuses | 2 x T4A, 250 V |
Footprint and dimensions
| Monitor | 10.1" LCD touchscreen |
|---|---|
| Footprint (w x d) | 387 mm x 289 mm (15.2" x 11.4") |
| Footprint with MiniSampler S (w x d) | 438 mm x 454 mm (17.2" x 17.9") |
| Dimensions (w x d) | 605 mm x 384 mm (23.8" x 15.1") |
| Dimensions with MiniSampler S (w x d) | 633 mm x 491 mm (24.9" x 19.3") |
| Dimensions with all covers open (w x d) | 819 mm x 610 mm (32.2" x 24.0") |
| Dimensions with accessory with all covers open (w x d) | 824 mm x 610 mm (32.4" x 24.0") |
| Height | 395 mm–500 mm (15.6"–19.7") (adjustable touchscreen) |
| Weight | 31 kg |
Column
The reusable autoMACS column is specifically designed for use with the autoMACS to deliver high cell purities and recoveries. The column is compatible with all starting materials and available MicroBeads and Isolation Kits. Once installed, the columns become part of a fluidic system that keeps the user safe when dealing with hazardous samples. Monitoring and priming of the column are automatically managed by the instrument software. A wizard guides through the column exchange step by step.
Column capacity
- Up to 4x109 total cells/sample
- Up to 2x108 labeled cells/sample
- Up to 28 mL whole blood
- Up to 28 mL buffy coat (circa 1/3 buffy coat from 500 mL whole blood)
Cell numbers and sample volume capacity
The autoMACS NEO Separator provides stage loading as a feature to exceed the maximum capacity of labeled cells within a single sample. During separation the instrument applies the sample onto the column in multiple aliquot portions, called stages, not exceeding the cell capacity of the column. Thus, larger samples can be processed from a single tube without aliquotation of a sample to several tubes. Find out which cell numbers and sample capacities can be processed in a single tube on the different Chill Rack sizes for your reagent of interest in the respective data sheets.
Principle
Any positive and untouched isolation can be performed to separate target cells from PBMCs, cultured cells, and dissociated tissue. In addition, cells can be directly isolated from whole blood or bone marrow. Overall more than 400 different reagents are available and we are always expanding our portfolio. Upon automated separation, labeled cells, as well as non-labeled cells, are obtained and can be used for downstream applications.
Separation strategies and technologies
Starting Material
| Sample type | PBMCs Whole blood Buffy coat Bone marrow Single-cell suspensions from dissociated (complex) tissue Cultured cells (cell lines and primary cells) |
|---|---|
| Species | Human Mouse Rat Non-human primate Any others are possible with indirect MicroBeads |
| Number of samples | 6 samples with 5 mL tubes 5 samples with 15 mL tubes 3 samples with 50 mL tubes |
Consumables
Accessories
- MACS MiniSampler S
- MACS Chill Racks: Chill 5 Rack, Chill 15 Rack, Chill 50 Rack
- MACS Chill Rack Sets, 3 racks: Chill 5, 15, 50 Rack Set (included); Chill 5 Rack Set; Chill 15 Rack Set; Chill 50 Rack Set
- MACS Reagent Rack 8 (included)
- autoMACS Laminar Hood Plate
Brochures/posters
Other downloads
We help you get the best results
- Our technical support team will assist you throughout your projects with experimental design, sample preparation, troubleshooting, and service options.
- You can find a collection of FAQs and technical documents, including datasheets, protocols, and manuals.
- Training at Miltenyi University
- Protect your instrument with a comprehensive service contract.
autoMACS® NEO Separator
Benchtop instrument for fully automated cell separation.
Catalog no.: 130-120-327
MiniSampler S
Catalog no.: 130-123-093
