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Discover our different workflows for microglia and find the one that fits your experimental needs:
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Immunofluorescence staining with neural cell antibodies for microscopy (protocol)
Neuroscience (brochure)
Tissue dissociation into single-cell suspensions with high cell viability and a minimum of cell debris is a prerequisite for reliable cell separation, cell culture, and flow cytometry analysis. A dissociation procedure that combines optimized enzymatic treatment using our Tissue Dissociation Kits with gentle mechanical dissociation (gentleMACS) is crucial for the preparation of viable single-cell suspensions.
Our MACS® MicroBead Technology, in combination with the Adult Brain Dissociation Kit provides a gentle technique with minimal manipulation time. It allows you to obtain pure, viable and functional adult microglia.
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5 benefits of using magnetic cell separation to isolate neural cells from the central nervous system
Read this recently published paper in PLoS ONE demonstrating the benefits of magnetic cell separation which include:
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