Analysis of SARS-CoV-2-specific B cells

Screening SARS-CoV-2-specific B cells can support the evaluation of efficacy of COVID-19 vaccines and also the prognosis of severe COVID-19 development. The SARS-CoV-2 B Cell Analysis Kit, human offers an easy and ready-to-use solution for detection, quantification and phenotyping of spike- and RBD-specific B cells circulating in peripheral blood.  

Find out more details in our online application protocol: Analysis of SARS-CoV-2–specific B cells using recombinant SARS-CoV-2 proteins
 

Enrichment of SARS-CoV-2-specific B cells 

Positive selection of SARS-CoV-2-specific B cells using the SARS-CoV-2 B Cell MicroBead Kit, human,  facilitates the enrichment of B cells specific for spike proteins or RBD.  

The several-fold enriched antigen-specific B cells can be used for: 

• expansion in culture
• molecular analysis, such as B cell receptor (BCR) cloning and sequencing
• single clone culture

Find out more details in our online application protocol: Enrichment of SARS-CoV-2–specific B cells using recombinant SARS-CoV-2 proteins

After staining with SARS-CoV-2 B Cell Analysis Kit, human  antigen-specific B cells, recognizing SARS-CoV-2 antigens, can be sorted using the MACSQuant Tyto Cell Sorter. These can be then used for further applications, including further expansion in culture, molecular analysis and single clone culture.

Find out more details in our online application protocol: Microchip-based cell sorting and expansion of SARS-CoV-2–specific B cells using the MACSQuant® Tyto® Cell Sorter

Sorting of antigen specific B cells using the MACSQuant® Tyto® 

Expansion of SARS-CoV-2-specific B cells

After enrichment SARS-CoV-2-specific B cells can be cultured using B Cell Expansion Kit, human.

The combination of CD40L, cross-linking antibody, and IL-4 included in the B Cell Expansion Kit allows you to expand the B cell population by several fold and obtain activated cells with a distribution of memory and plasma cells similar to the original fraction.

After expanding SARS-CoV-2-specific B cells, SARS-CoV-2-specific IgG antibodies can be detected in the supernatant, confirming that the B Cell Expansion Kit supports the expansion of fully functional antibody-secreting cells, suitable for applications such as monoclonal antibody discovery and generation.