The NK Cell Isolation Kit was developed for the isolation of untouched NK cells from single-cell suspensions of murine spleen. Non-NK cells, i.e. T cells, dendritic cells, B cells, granulocytes, macrophages, and erythroid cells are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled NK cells is achieved by depletion of non-target cells. The kit has been optimized to give outstanding purities in C57BL/6J mice.

Data and images for NK Cell Isolation Kit, mouse

Figures

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
A) BALB/c mouse spleen cell suspension
Before separation
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
Isolated NK cells
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
B) C57BL/6 mouse spleen cell suspension
Before separation
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
Isolated NK cells
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.
View details

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.

Specifications for NK Cell Isolation Kit, mouse

Overview

The NK Cell Isolation Kit was developed for the isolation of untouched NK cells from single-cell suspensions of murine spleen. Non-NK cells, i.e. T cells, dendritic cells, B cells, granulocytes, macrophages, and erythroid cells are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled NK cells is achieved by depletion of non-target cells. The kit has been optimized to give outstanding purities in C57BL/6J mice.

Detailed product information

Downstream applications

NK cells isolated using the NK Cell Isolation Kit can be used for
in vitro
and
in vivo
applications, e.g., functional studies or adoptive transfer experiments.

Columns

MS, LS, or autoMACS
®
Columns.

Resources for NK Cell Isolation Kit, mouse

Documents and Protocols

Related products for
NK Cell Isolation Kit, mouse

4 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?