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Miltenyi Biotec-tested panel 18 (MBTP 18)
This application protocol describes the flow cytometric analysis of NK cells after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD11b||REA592||NK cell maturation||VioBlue®||450/50 (violet)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|CD335 (NKp46)||REA815||NK cells||FITC||525/50 (blue)|
|KLRG1||REA1016||Mature NK cells||PE||585/40 (blue)|
|CD3e||REA606||Cell type exclusion||PE-Vio® 615||655-730 (blue)|
|CD19||REA749||Cell type exclusion||PE-Vio 615||655-730 (blue)|
|NK1.1||PK136*||NK cells||PE-Vio 770||750LP (blue)|
|CD27||REA499||NK cell maturation||APC||655-730 (red)|
|CD45||REA737||Leukocytes||APC-Vio 770||750LP (red)|
*alternative REAfinity™ Antibody available: NK1.1 Antibody, anti-mouse, PE-Vio 770, REAfinity
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