Immunophenotyping of human CD4+ and CD8+ T cell activation and differentiation using flow cytometry (panel A)

MBTP 56 = Miltenyi Biotec-tested panel 56

This application protocol describes the analysis of human CD4+ and CD8+ T cell activation and differentiation markers after T cell stimulation. To demonstrate this state-of-the-art panel for T cell activation as well as T cell differentiation of both CD4 T cells and CD8 T cells, PBMCs were stimulated for 48 hours using T Cell TransAct™, human. The state of activation and differentiation was defined by CD45RA, CD25, CD69, and CD197 (CCR7) expression.

Protocol

Antibody panel for T cell activation and differentiation

SpecificityClonePurposeFluorochromeDetection filter (nm) (laser)
7-AAD*-Viability-655-730 (blue)
CD45RAREA562T cell activation and differentiationFITC525/50 (blue)
CD4*REA623Helper T cellsVioGreen™525/50 (violet)
CD8*REA734Cytotoxic T cellsAPC-Vio® 770750 LP (red)
CD197 (CCR7)REA108T cell activation and differentiationAPC655-730 (red)
CD3REA613T cells VioBlue®450/50 (violet)
CD25 REA570T cell activation and differentiationPE 585/40 (blue)
CD69REA824T cell activation and differentiationPE-Vio 770750 LP (blue)

* Alternative CE-IVD/ASR reagents available.

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Materials

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