The T Cell Activation/Expansion Kit was developed to activate and expand rhesus monkey T cells.

Specifications for T Cell Activation/Expansion Kit, non-human primate

Overview

The T Cell Activation/Expansion Kit was developed to activate and expand rhesus monkey T cells.

Detailed product information

Background information

The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against non-human primate CD3, and human CD2 and CD28. Anti-Biotin MACSiBead™ Particles loaded with biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells from PBMCs as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 14 of culture.

Downstream applications

Activated T cells can be used for many downstream applications, such as cytokine analysis or immunoprecipitation, and cells can also be transfected with high efficiency.

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