Clone:
4E3
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα

Extended validation for CD25 Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD25. Human peripheral blood mononuclear cells (PBMCs) were stained with CD25 antibodies and with a suitable counterstaining. As a control, CD25 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD25 (4E3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD25 (4E3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD25 (4E3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD25 Antibody, anti-human

Overview

The CD25 antibody recognizes the human CD25 antigen, a 28 kDa glycoprotein, also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4
+
regulatory T cells.
The CD25 antigen contains three epitope regions called A, B, and C. This CD25 antibody recognizes epitope region B.

Alternative names

IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα

Detailed product information

Technical specifications

Clone4E3
Clonalitymonoclonal
Isotypemouse IgG2bκ
Isotype controlIsotype Control Antibody, mouse IgG2b
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD25
Alternative names of antigenIL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα
Molecular mass of antigen [kDa]28
Distribution of antigenT cells, B cells, NK cells, lymphocytes, macrophages, monocytes, osteoblasts, thymocytes, basophils
Entrez Gene ID3559
RRIDAB_2734062, AB_2733789, AB_2733790, AB_2726345, AB_2726068, AB_2733510, AB_2733511, AB_2734061

Resources for CD25 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD25 Antibody, anti-human

Publications

  1. Sakaguchi, S. et al. (1995) Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor α-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J. Immunol. 155: 1151-1164
  2. Lamprecht, B. et al. (2008) Aberrant expression of the Tʜ2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha. Blood 112(8): 3339-3347
  3. Maloy, K. J. et al. (2001) Regulatory T cells in the control of immune pathology. Nat. Immunol. 2(9): 816-822
  4. Shevach, E. M. (2001)
    Certified professionals: CD4
    +
    CD25
    +
    suppressor T cells.
    J. Exp. Med. 193(11): F41-F46
  5. Chaput, N. et al. (2013)
    Phase I clinical trial combining imatinib mesylate and IL-2: HLA-DR
    +
    NK cell levels correlate with disease outcome.
    Oncoimmunology 2(2): e23080

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