Neuronal cells

Isolation and cultivation of mouse neurons and neuronal precursor cells

  • Brain tissue dissociation with minimum damage to synapses, axons, and dendrites
  • Neuron isolation from neonatal and adult mouse brain 
  • Primary neuronal cell cultivation in a serum-free and feeder layer–free system

Application protocols

Discover our different workflows for neuronal cells and find the one that fits your experimental needs.

Isolation and cultivation of neurons from neonatal mouse brain

Isolation and cultivation of neurons from adult mouse brain

Isolation and cultivation of CD171 (L1CAM) positive neurons from neonatal mouse brain
 

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Brain (mouse)

Neural cells (mouse)

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Webinar
MACS Academy: Adult brain dissociation and neural cell isolation

Join Dr. Hui Zhang and her webinar on the latest options and integrated solutions for neuroscience research and the use of MACS Technology for adult brain dissociation and neural cell isolation.

Application data by workflow step

Murine brain tissue dissociation

Purity and viability of cells before and after sample clearing
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Highly viable and pure adult neural cells

Cleared cell suspensions obtained with the Adult Brain Dissociation Kit, mouse and rat contain noticeable less cell debris and erythrocytes. The viable and pure cells are ready for subsequent cell isolation, cultivation, and analysis.

Purity of cell suspensions obtained from murine (P4) whole brain tissue
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Highly viable and pure postnatal neural cells

Whole brain tissue from P4 mice dissociated with the Neural Tissue Dissociation Kit (P) results in a high yield of viable cells.

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 Isolation of untouched neurons

Analysis of single-cell suspensions from neonatal and adult brain tissue before and after neuron isolation
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Rapid isolation of untouched neurons from neonatal and adult mouse brain 

Untouched neonatal and adult neurons can be purified by magnetically labeling and depleting undesired cells with the Neuron Isolation Kit, mouse.

The untouched isolation is simple and fast. It takes only one hour to acquire highly purified and viable neurons from a single cell suspension.

The high purity of the isolated neurons relies on the specificity of the non-neuronal cell surface marker used in the Neuron Isolation Kit, mouse.

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Scientific poster
Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol

Hui Zhang1, Sandy Reiß1, Stefan Tomiuk1, Silvia Rüberg1, Richard Fekete2, Melanie Jungblut1, and Andreas Bosio1

1Miltenyi Biotec GmbH, Bergisch Gladbach, Germany; 2Fluidigm Corporation, South San Francisco, CA, USA

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Scientific poster
A novel method for rapid isolation of “untouched” neurons by immunomagnetic depletion of non-neuronal cells 

Melanie Jungblut, Ina Herzig, and Andreas Bosio

Miltenyi Biotec GmbH, Bergisch Gladbach, Germany

MACS Handbook:

Neural cells (mouse)

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Cultivation of neurons from mouse brain tissue

Staining of cultured primary adult mouse neurons
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Improved growth and survival

Cultivation of primary adult mouse neurons can be a challenging task. MACS® NeuroBrew®-21 supplement has been designed for optimal growth and long term viability of neural cells of the central and peripheral nervous system. Experience the growth of nearly pure populations of neural cells in culture without the need of an astrocyte feeder layer.

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Immunofluorescence staining and microscopy analysis of neuronal cells

Neural cell–specific monoclonal antibodies allow a fast and easy way to perform immunofluorescent staining for subsequent microscopy analysis. Benefit from short primary antibody incubation and the possibility to stain PFA-fixed neural tissue cryosections and adherent or fixed neural cells in vitro.

CD171 staining of adult mouse neuronal cells
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Neuronal cell staining targeting CD171

Immunofluorescence staining of the neuronal cell marker CD171, also known as L1CAM (L1 cell adhesion molecule).

Anti-PSA-NCAM staining of mouse neuronal cells
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Anti-PSA-NCAM staining of neuronal cells

Microscopy analysis of cultured neuronal precursor cells and cryosections of adult mouse brain after anti-PSA-NCAM staining.


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