MACS NeuroBrew-21 are serum-free neural supplements designed for use in combination with MACS Neuro Medium for the
in vitro
propagation of mature neural cells of the central and peripheral nervous system, or neural stem cells of primary or ES/iPS cell origin.

Data and images for
MACS
®
NeuroBrew
®
-21

Figures

Figure 1

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Light microscopic picture of neurosphere formation after 7 days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (P) and magnetically isolated using Anti-Prominin-1 MicroBeads prior to cell culture.

Figure 1

Light microscopic picture of neurosphere formation after 7 days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (P) and magnetically isolated using Anti-Prominin-1 MicroBeads prior to cell culture.

Figure 2

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Light microscopic picture of PSA-NCAM–positive neuronal precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

Figure 2

Light microscopic picture of PSA-NCAM–positive neuronal precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

Figure 3

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Light microscopic picture of A2B5-positive glial precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. A2B5-positive cells were processed for immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a marker for glial precursor cells.
Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

Figure 3

Light microscopic picture of A2B5-positive glial precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. A2B5-positive cells were processed for immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a marker for glial precursor cells.
Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

Specifications for
MACS
®
NeuroBrew
®
-21

Overview

MACS NeuroBrew-21 are serum-free neural supplements designed for use in combination with MACS Neuro Medium for the
in vitro
propagation of mature neural cells of the central and peripheral nervous system, or neural stem cells of primary or ES/iPS cell origin.

Detailed product information

Background information

MACS NeuroBrew-21 is a re-optimized formulation of the B27 components designed to improve performance.
1
It has been developed for the
in vitro
propagation of neural cells of the central and peripheral nervous system.
MACS NeuroBrew-21 has been designed for
  • optimal growth and long term viability of neural cells of the central and peripheral nervous system
  • the growth of nearly pure populations of neural cells without the need of an astrocyte feeder layer
MACS NeuroBrew-21 w/o Vitamin A is identical to MACS NeuroBrew-21, but without the addition of Vitamin A or other retinoid related components. Vitamin A induces the differentiation of neural stem cells into mature neural cells. MACS NeuroBrew-21 w/o Vitamin A is suitable for use with studies of neural stem cells, neural development and neural cell differentiation.
MACS NeuroBrew-21 w/o Vitamin A has been designed for use with
  • primary neural stem or progenitor cells
  • primary neurospheres
  • ES/iPS cell derived neural stem cells and neurosphere

References for
MACS
®
NeuroBrew
®
-21

Publications

  1. Chen, Y. et al. (2008) NS21: re-defined and modified supplement B27 for neuronal cultures. J. Neurosci. Methods 171: 239-247

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®
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