Retroviral transduction of hematopoietic stem cells (HSCs)

Application protocols

Discover our different hematopoietic stem cell workflows and find the one that fits your experimental needs.
 

Viral transduction of hematopoietic cells is a fast, efficient method commonly used to study gene function, modulate gene expression,  or deliver a potential vector for gene correction in gene therapies. Vectofusin-1 is a fully synthetic, non-toxic cationic amphipathic peptide with viral transduction enhancing capacity, enabling high transduction levels of HSCs with low amounts of retroviral vector. Download the scientific poster below for more detailed information:

StemMACS™ HSC Expansion Media XF enables robust expansion of hematopoietic stem cells from cord blood, bone marrow, or peripheral blood. Combined with the StemMACS HSC Expansion Cocktail, a set of cytokines, this xeno-free culture medium can be used to preserve high levels of primitive CD34⁺CD133⁺ hematopoietic cells. 

Flow cytometric analyses provide you with quantitative information about the expression of intracellular as well as surface markers and let you assess the efficiency and quality of your viral transduction in just a few simple steps.