Clone:
AC136
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ, mouse IgG2a
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
gp105-120, Mucosialin, My10

Extended validation for CD34 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with AC136
561++
581++
563++
8G12++
4H11++
QBEND/10+
REA1164++
Cells were incubated with an excess of purified unconjugated CD34 (AC136) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD34 (AC136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD34 (AC136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD34 (AC136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD34 Antibody, anti-human

Overview

The monoclonal antibody clone AC136 detects a class III epitope of the CD34 antigen. This epitope is different than the one recognized by the clone used in the CD34 MicroBead Kits.
The CD34 antigen is a single chain transmembrane glycoprotein, expressed on human hematopoitetic stem and progenitor cells, endothelial progenitor cells, vascular endothelial cells, embryonic fibroblasts, and some cells in fetal and adult nervous tissue. The antigen is absent on fully differentiated hematopoietic cells such as normal peripheral blood lymphocytes, monocytes, granulocytes, erythrocytes, and platelets. Clone AC136 has a similar specifity as the CD34 monoclonal antibody clone 8G12 (HPCA-2).
CD34 antibodies can be used for studies of hematopoiesis and nonhematopoiectic stem cells, phenotyping of hematopoietic stem cells, and studies on phenotyping of hematologic malignancies, endothelial cells, and endothelial progenitor cells (EPCs).

Alternative names

gp105-120, Mucosialin, My10

Detailed product information

Technical specifications

CloneAC136
Clonalitymonoclonal
Isotypemouse IgG2aκ, mouse IgG2a
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD34
Alternative names of antigengp105-120, Mucosialin, My10
Molecular mass of antigen [kDa]37
Distribution of antigenstem cells, chondrocytes, dendritic cells, endothelial cells, fibroblasts, mast cells, osteoblasts, cancer stem cells, CNS cells, hematopoietic stem and progenitor cells, bone marrow, liver, dendritic cells, endothelial cells, fibroblasts, osteoblasts, mast cells, stem cells, cancer stem cells, hematopoietic stem and progenitor cells, bone marrow, liver
Entrez Gene ID947
RRIDAB_2726005, AB_2726281, AB_2726006, AB_2726278, AB_2726003, AB_2726283, AB_2726008, AB_2726284, AB_2726009, AB_2733225, AB_2733226, AB_2726282, AB_2726007, AB_2726279, AB_2726004, AB_2660378, AB_2726280

Resources for CD34 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD34 Antibody, anti-human

Excellent CD34 Antibody from Miltenyi Biotec

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CD34-FITC, human (130-113-178)

Will buy and use it again. Recommendable!

References for CD34 Antibody, anti-human

Publications

  1. De Paepe, M. E. et al. (2011) Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells. Am. J. Pathol. 178(3): 1329-1339
  2. Stull, R. A. et al. (2000) Simultaneous flow cytometric analyses of enhanced green and yellow fluorescent Cytometry 40: 126-134
  3. Vets, S. et al. (2012) Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model. Mol. Ther. 20(5): 908-917
  4. Bility, M. T. et al. (2012) Generation of a humanized mouse model with both human immune system and liver cells to model hepatitis C virus infection and liver immunopathogenesis. Nat. Protoc. 7(9): 1608-1617
  5. Metsuyanim, S. et al. (2009) Expression of stem cell markers in the human fetal kidney. PLoS One 4(68): e6709
  6. Lian, W. et al. (2014)
    Varying levels of 6-keto-prostaglandin F1α and thromboxane B2 in serum and endothelialization and hyperplasia in small-diameter grafts seeded with CD34
    +
    bone marrow cells in canines.
    Exp Ther Med. 7(5): 1123-1129
  7. Liu, H. et al. (2013) Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis 4: e857

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