StraightFrom
®
Whole Blood CD3 MicroBeads were developed for rapid positive selection of CD3
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Data and images for
StraightFrom
®
Whole Blood CD3 MicroBeads
, human

Figures

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD3 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD3 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD3 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
StraightFrom
®
Whole Blood CD3 MicroBeads
, human

Overview

StraightFrom
®
Whole Blood CD3 MicroBeads were developed for rapid positive selection of CD3
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Detailed product information

Background information

StraightFrom
®
Whole Blood CD3 MicroBeads recognize the CD3 antigen which is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes. StraightFrom Whole Blood MicroBeads allow the fast isolation of CD3
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS
®
Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD3 MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

The purified CD3
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
1,2
.

Columns

autoMACS
®
Columns or Whole Blood Columns.

References for
StraightFrom
®
Whole Blood CD3 MicroBeads
, human

Publications

  1. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
  2. Adams, S. et al. (2004) Cell lineage-specific chimerism in posthematopoietic stem cell transplant patients. MACS&more 8(2): 16
  3. Willasch et al. (2010) Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis Bone Marrow Transplant. 45: 181-189
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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StraightFrom
®
Whole Blood CD3 MicroBeads
, human

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