Clone:
5B1
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ, mouse IgG2a
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
Ptprc, T200, LCA, LY5, L-CA

Extended validation for CD45 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 5B1
REA747++
2D1++
HI30++
MB4-6D6-
REA1023-
REAL258++
Cells were incubated with an excess of purified unconjugated CD45 (5B1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD45. Human peripheral blood mononuclear cells (PBMCs) were stained with CD45 antibodies and plotted against the side scatter. As a control, CD45 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45. Human peripheral blood mononuclear cells (PBMCs) were stained with CD45 antibodies and plotted against the side scatter. As a control, CD45 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45. Human peripheral blood mononuclear cells (PBMCs) were stained with CD45 antibodies and plotted against the side scatter. As a control, CD45 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45. Human peripheral blood mononuclear cells (PBMCs) were stained with CD45 antibodies and plotted against the side scatter. As a control, CD45 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45. Human peripheral blood mononuclear cells (PBMCs) were stained with CD45 antibodies and plotted against the side scatter. As a control, CD45 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45. Human peripheral blood mononuclear cells (PBMCs) were stained with CD45 antibodies and plotted against the side scatter. As a control, CD45 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD45. Human peripheral blood mononuclear cells (PBMCs) were stained with CD45 antibodies and plotted against the side scatter. As a control, CD45 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD45 (5B1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD45 (5B1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD45 (5B1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD45 Antibody, anti-human

Overview

The CD45 antibody recognizes the human CD45 antigen, a tyrosine phosphatase also known as leukocyte common antigen (LCA). The CD45 molecule is required for T and B cell activation and is expressed in at least five isoforms depending on the differentiation status of the cell. The CD45 antibody recognizes a common epitope of all CD45 isoforms.

Alternative names

Ptprc, T200, LCA, LY5, L-CA

Detailed product information

Technical specifications

Clone5B1
Clonalitymonoclonal
Isotypemouse IgG2aκ, mouse IgG2a
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD45
Alternative names of antigenPtprc, T200, LCA, LY5, L-CA
Molecular mass of antigen [kDa]145
Distribution of antigenB cells, dendritic cells, granulocytes, stem cells, Langerhans cells, macrophages, mast cells, monocytes, T cells, leukocytes, lymphocytes, basophils, hematopoietic stem and progenitor cells, plasma cells, thymocytes
Entrez Gene ID5788
RRIDAB_2725945, AB_2726226, AB_2725951, AB_2726699, AB_2726221, AB_2725946, AB_2726217, AB_2725942, AB_2726225, AB_2725950, AB_2726227, AB_2725952, AB_2726223, AB_2725948, AB_2726222, AB_2725947, AB_2726218, AB_2725943, AB_2726224, AB_2725949, AB_2726219, AB_2725944, AB_2660435, AB_2726220

Resources for CD45 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD45 Antibody, anti-human

Excellent CD45 Antibody from Miltenyi Biotec

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CD45-FITC, human (130-113-117)

Will buy and use it again. Recommendable!

Staining Peripheral Blood with Anti-CD45 VioBlue

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CD45-VioBlue, human (130-113-122)

Works well. Reproducible results.

Staining Peripheral Blood with Anti-CD45 VioBlue

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CD45-VioBlue, human (130-113-684)

Works well. Reproducible results.

References for CD45 Antibody, anti-human

Publications

  1. Kurian, L. et al. (2013) Conversion of human fibroblasts to angioblast-like progenitor cells. Nat. Methods 10(1): 77-83
  2. Vykoukal, J. et al. (2008) Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation. Lab Chip 8(8): 1386-1393

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