Clone:
REA1037
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MC
Alternative names:
BSF2, HGF, HSF, IFNB2

Extended validation for IL-6 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1037
MQ2-13A5++
AS12-
MQ2-6A3-
Cells were incubated with an excess of purified unconjugated IL-6 (REA1037) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for IL-6. Human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and Brefeldin A for 6h were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with IL-6 antibodies. As a control, IL-6 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-6. Human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and Brefeldin A for 6h were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with IL-6 antibodies. As a control, IL-6 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-6. Human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and Brefeldin A for 6h were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with IL-6 antibodies. As a control, IL-6 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-6. Human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and Brefeldin A for 6h were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with IL-6 antibodies. As a control, IL-6 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for IL-6. Human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and Brefeldin A for 6h were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with IL-6 antibodies. As a control, IL-6 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for IL-6 Antibody, anti-human, REAfinity™

Overview

Clone REA1037 recognizes the Interleukin 6 (IL-6) antigen, which is originally identified as a B cell differentiation factor Il-6 is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, fibroblasts, endothelial cells, eosinophils, and T cells. Disturbed IL-6 production has been associated with pathological processes, including inflammatory autoimmune diseases and cancer.
Additional information: Clone REA1037 displays negligible binding to Fc receptors.

Alternative names

BSF2, HGF, HSF, IFNB2

Detailed product information

Technical specifications

CloneREA1037
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenIL-6
Alternative names of antigenBSF2, HGF, HSF, IFNB2
Molecular mass of antigen [kDa]21
Distribution of antigenmonocytes, fibroblasts, endothelial cells, T cells, eosinophils
Entrez Gene ID3569
RRIDAB_2727948, AB_2728000, AB_2727949, AB_2728001, AB_2727950, AB_2728002, AB_2727951, AB_2819611, AB_2727999

Resources for IL-6 Antibody, anti-human, REAfinity™

Certificates

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