The Inside Stain Kit contains reagents for the fixation and permeabilization of cells for intracellular staining, for example for flow cytometric evaluation. The kit was also optimized to function in combination with MACS
®
Technology.
Typical applications of the Inside Stain Kit include:
  • Intracellular staining of cytokine producing cells for cytokine detection by flow cytometry
  • Intracellular staining of enriched cells, e.g., CD138+ cells for detection of intracellular kappa/lambda light chains, or melanoma cells

Data and images for Inside Stain Kit

Figures

Figure 1

CD138
+
plasma cells isolated from PBMCs of a healthy human donor were stained with Anti-kappa-light-chain-FITC, CD138-PE, and Anti-lambda-light-chain-Cy®5 using the Inside Stain Kit by solid-phase intracellular staining in combination with MACS Technology.
A:
B:
View details

Figure 1

CD138
+
plasma cells isolated from PBMCs of a healthy human donor were stained with Anti-kappa-light-chain-FITC, CD138-PE, and Anti-lambda-light-chain-Cy®5 using the Inside Stain Kit by solid-phase intracellular staining in combination with MACS Technology.
View details

Figure 1

CD138
+
plasma cells isolated from PBMCs of a healthy human donor were stained with Anti-kappa-light-chain-FITC, CD138-PE, and Anti-lambda-light-chain-Cy®5 using the Inside Stain Kit by solid-phase intracellular staining in combination with MACS Technology.

Figure 2

View details
Solid-phase intracellular staining technology in combination with MACS Technology.

Figure 2

Solid-phase intracellular staining technology in combination with MACS Technology.

Specifications for Inside Stain Kit

Overview

The Inside Stain Kit contains reagents for the fixation and permeabilization of cells for intracellular staining, for example for flow cytometric evaluation. The kit was also optimized to function in combination with MACS
®
Technology.
Typical applications of the Inside Stain Kit include:
  • Intracellular staining of cytokine producing cells for cytokine detection by flow cytometry
  • Intracellular staining of enriched cells, e.g., CD138+ cells for detection of intracellular kappa/lambda light chains, or melanoma cells for detection of Melan A expression
  • Intracellular staining of other cells enriched using MACS Technology

Detailed product information

Background information

The Inside Stain Kit and the newly developed protocol for solid-phase intracellular staining allow the fast, easy, and efficient staining of cells while they are immobilized within a MACS Column during separation. Thus, extra centrifugation steps are unnecessary, minimizing cell loss. Typically, separated cells eluted from the first column are fixed in suspension by adding Inside Fix. After direct application of the cells to a second column, permeabilization and staining reagents are added. The column is then removed from the magnetic field and the stained cells are eluted and directly analyzed. This solid-phase, intracellular staining requires only very small volumes of staining reagents. Optimal staining in combination with MACS Technology can be achieved on MS Columns.

Columns

For enrichment and in-column staining: MS Columns.

References for Inside Stain Kit

Publications

  1. Scheffold, A: et al. (2018) CD137+CD154- Expression As a Regulatory T Cell (Treg)-Specific Activation Signature for Identification and Sorting of Stable Human Tregs from In Vitro Expansion Cultures. Front Immunol 9
  2. Horst, A. et al. (2002)
    Detection and characterization of plasma cells in peripheral blood: correlation of IgE
    +
    plasma cell frequency with IgE serum titre.
    Clin. Exp. Immunol. 130: 370-378