CD25 MicroBeads II were developed for positive selection or depletion of CD25
+
cells.

Data and images for CD25 MicroBeads II, human

Figures

Figure 1

Depletion of CD25
+
cells from PBMCs using CD25 MicroBeads II, an LD Column, and a MidiMACS™ Separator.
PBMCs before separation
CD25
+
cell depleted fraction
View details

Figure 1

Depletion of CD25
+
cells from PBMCs using CD25 MicroBeads II, an LD Column, and a MidiMACS™ Separator.
View details

Figure 1

Depletion of CD25
+
cells from PBMCs using CD25 MicroBeads II, an LD Column, and a MidiMACS™ Separator.

Specifications for CD25 MicroBeads II, human

Overview

CD25 MicroBeads II were developed for positive selection or depletion of CD25
+
cells.

Detailed product information

Background information

CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4
+
T cells, which has regulatory function.
The CD25 MicroBeads II recognize epitope A of the CD25 molecule.
For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS
®
CD25 Antibodies, which bind to epitope B of the CD25 antigen.
For isolation of CD25
+
cell subsets, the CD25 MicroBeads II can be combined with MACS
®
Cell Isolation Kits or MultiSort Kits. The CD4
+
CD25
+
Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4
+
CD25
+
regulatory T cells.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

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