Clone:
REA187
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS
Alternative names:
IGF2R, CIMPR, M6P-R, MPR1, MPRI

Extended validation for CD222 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD222 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD222-PE (REA187, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD222 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD222-PE (REA187, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD222 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD222-PE (REA187, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD222 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD222-PE, clone (REA187). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD222 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD222-PE, clone (REA187). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD222 Antibody, anti-human, REAfinity™

Overview

Clone REA187 recognizes CD222, a 300 kDa type I transmembrane protein, which serves as a multifunction receptor and is ubiquitously expressed. CD222 has a large extracytoplasmic domain, a single transmembrane region, and a short cytoplasmic tail that lacks intrinsic catalytic activity. The receptor cycles continuously between intracellular compartments and the plasma membrane. At steady state, CD222 is found mainly intracellularly, and to a lesser extent on the cell surface. CD222 binds IGF-2 and mannose-6 phosphate containing ligands such as transforming growth factor‐β, leukemia inhibitory factor, and proliferin. This interaction allows CD222-mediated regulation of ligand dependent signaling and uptake and cellular processing of ligands. CD222 is further suggested to function as the cation-independent cell surface receptor for heparanase. Interaction of heparanase with various cell surfaces is suggested to be important for heparanse functions. Cell surface displayed heparanase allows efficient degaradation of local extracellular matrix (ECM)/basement membrane (BM) components.
Additional information: Clone REA187 displays negligible binding to Fc receptors.

Alternative names

IGF2R, CIMPR, M6P-R, MPR1, MPRI

Detailed product information

Technical specifications

CloneREA187
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
AntigenCD222
Alternative names of antigenIGF2R, CIMPR, M6P-R, MPR1, MPRI
Distribution of antigenB cells, T cells, endothelial cells
RRIDAB_2656392, AB_2656393, AB_2656394, AB_2656395, AB_2656396, AB_2656397, AB_2656398, AB_2656399, AB_2656400, AB_2656401, AB_2656402, AB_2656403, AB_2656404, AB_2656391

Resources for CD222 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD222 Antibody, anti-human, REAfinity™

Publications

  1. Leksa, V. et al. (2012) Dissecting mannose 6-phosphate-insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J. Biol. Chem. 287: 22450-22462
  2. Wood, R. J. et al. (2008) Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. J. Biol. Chem. 283: 4165-4176

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