Name: Plasma Cell Isolation Kit II, human
Availability: InStock

Product data and images for Plasma Cell Isolation Kit II, human

Figures

Figure 1

CD38

⁺

plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.

Bone marrow before separation	Pre-enriched plasma cells after depletion of non-plasma cells
View details Figure 1 CD38 ⁺ plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.	View details Figure 1 CD38 ⁺ plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.
Enriched CD38+ plasma cells
View details Figure 1 CD38 ⁺ plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.

Specifications for Plasma Cell Isolation Kit II, human

Overview

The Plasma Cell Isolation Kit II has been developed for the isolation of plasma cells from human PBMCs or bone marrow.

Detailed product information

Background information

Human plasma cells are a heterogenous cell population comprising several subsets from short-lived and proliferative plasmablasts in lymphoid tissues, transitional plasma cells in peripheral blood to long-lived and non-dividing plasma cells in bone marrow

^1–3

. Plasma cells of all differentiation stages are identified by the expression of high levels of the CD38 antigen. Other surface markers, however, are differentially regulated dependent on the stage of differentiation and the spatial localization. Plasma cells in the blood circulation lack the expression of the typical B cell marker CD22 and were found to express lower levels of CD19 than mature B cells.

They are further characterized as being CD27

⁺⁺

, CD31

⁺

, CD44

⁺

, CD45

⁺

, CD56

^–

, CD62L

⁺

, CD86

⁺

, and HLA-DR

⁺

^1,4

A subset of CD38

⁺

blood plasma cells further expresses the CD138 antigen, whereas all CD38

⁺

plasma cells are also CD138

⁺

in bone marrow.

⁴

For the isolation of malignant plasma cells from bone marrow, the addition of CD56 MicroBeads may be left aside to avoid depletion of CD56-expressing tumor cells.

Detailed separation procedure

Non-plasma cells are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted by separation over a MACS Column. In the second step, pre-enriched plasma cells (flow-through fraction) are directly labeled with CD38 MicroBeads and isolated by positive selection from the pre-enriched plasma cell fraction.

Downstream applications

Isolated CD38

⁺

plasma cells can be used for the analysis of signal transduction pathways and molecular analysis, for gene expression profiling, studies on plasma cell dysfunctions (in allergy, asthma, autoimmunity, or infectious diseases) or studies on the migrational behavior of plasma cells.

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

References for Plasma Cell Isolation Kit II, human

Publications

Medina et al. (2002) The heterogeneity shown by human plasma cells from tonsil, blood, and bone marrow reveals graded stages of increasing maturity, but local profiles of adhesion molecule expression. Blood 99: 2154-2161
Slifka, M. K. et al. (1998) Humoral immunity due to long-lived plasma cells. Immunity 8: 363-372
Manz, R. A. et al. (1997) Lifetime of plasma cells in the bone marrow. Nature 388: 133-134
Horst, A. et al. (2002)
Detection and characterization of plasma cells in peripheral blood: correlation of IgE
⁺
plasma cell frequency with IgE serum titre.
Clin. Exp. Immunol. 130: 370-378

Data and images

Data and images for Plasma Cell Isolation Kit II, human

Figures

Figure 1

CD38

⁺

Bone marrow before separation	Pre-enriched plasma cells after depletion of non-plasma cells
View details Figure 1 CD38 ⁺ plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.	View details Figure 1 CD38 ⁺ plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.
Enriched CD38+ plasma cells
View details Figure 1 CD38 ⁺ plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.

Specifications

Specifications for Plasma Cell Isolation Kit II, human

Overview

The Plasma Cell Isolation Kit II has been developed for the isolation of plasma cells from human PBMCs or bone marrow.

Detailed product information

Background information

^1–3

They are further characterized as being CD27

⁺⁺

, CD31

⁺

, CD44

⁺

, CD45

⁺

, CD56

^–

, CD62L

⁺

, CD86

⁺

, and HLA-DR

⁺

^1,4

A subset of CD38

⁺

blood plasma cells further expresses the CD138 antigen, whereas all CD38

⁺

plasma cells are also CD138

⁺

in bone marrow.

⁴

For the isolation of malignant plasma cells from bone marrow, the addition of CD56 MicroBeads may be left aside to avoid depletion of CD56-expressing tumor cells.

Detailed separation procedure

Downstream applications

Isolated CD38

⁺

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

References

References for Plasma Cell Isolation Kit II, human

Publications

Medina et al. (2002) The heterogeneity shown by human plasma cells from tonsil, blood, and bone marrow reveals graded stages of increasing maturity, but local profiles of adhesion molecule expression. Blood 99: 2154-2161
Slifka, M. K. et al. (1998) Humoral immunity due to long-lived plasma cells. Immunity 8: 363-372
Manz, R. A. et al. (1997) Lifetime of plasma cells in the bone marrow. Nature 388: 133-134
Horst, A. et al. (2002)
Detection and characterization of plasma cells in peripheral blood: correlation of IgE
⁺
plasma cell frequency with IgE serum titre.
Clin. Exp. Immunol. 130: 370-378

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Plasma Cell Isolation Kit II, human

Plasma Cell Isolation Kit II, human

Product overview

Product data and images for Plasma Cell Isolation Kit II, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for Plasma Cell Isolation Kit II, human

Overview

Detailed product information

Background information

Detailed separation procedure

Downstream applications

Columns

References for Plasma Cell Isolation Kit II, human

Publications

Data and images

Data and images for Plasma Cell Isolation Kit II, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for Plasma Cell Isolation Kit II, human

Overview

Detailed product information

Background information

Detailed separation procedure

Downstream applications

Columns

References

References for Plasma Cell Isolation Kit II, human

Publications

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