Solutions for stimulation, enrichment, and analysis of rare virus-specific T cells 

Antigen-specific T cells play a pivotal role in fighting viral infections. Assessment of specificity, phenotype, and functional cytokine secretion of virus-activated T cells provides essential information on infection progression and the reactivity of the immune system.

However, assessment of ag-specific T cells is challenging as they constitute an extremely rare subset. Thus, a precise and sensitive analytic means is indispensable. 

Miltenyi Biotec offers several innovative products, protocols, and webinars to support you at every step of your workflow and accelerate your research:

In vitro stimulation of  T cells with peptide pools

  • PepTivator Peptide Pools enable the efficient virus-specific stimulation of CD4+ and CD8+ T cells of interest. Soon available for SARS-CoV-2.

Enrichment of antigen-specific T cells

Flow analysis of antigen-specific T cells

  • Rapid Cytokine Inspector Kits are unique tools that combine surface marker and intracellular cytokine staining in an easy and fast way, while allowing precise analyses.
  • MACSplex Assays can be used if you are interested in analysis of multiple soluble cytokines in one sample.
  • MACS® Cytokine Secretion Assays are kits that enable you to detect and enrich viable cytokine-screening T cells at the level of one in a million, which is especially useful when viable antigen-specific T cells are needed for further down-stream applications.
  • The 8-Color Immunophenotyping Kit, human is a pre-titrated antibody cocktail which offers the most reliable immunophenotyping in a variety of samples. This enables you to identify human immune cell subsets such as monocytes, neutrophils, eosinophils, T cells, B cells, and NK cells in one sample – quickly, easily, and reproducibly.

REAfinity antibodies for flow analysis of  T cells

For research requiring isolation of Pan T cells and T cell subsets

Untouched isolation of T cells and T cell subsets 

Direct isolation of T cells from blood and blood products

StraightFrom MicroBeads allow direct isolation of T cells from whole blood and blood products (Buffy Coat, LeukoPak® and LRSC) for e.g.  antigen-specific presentation in a Mixed Lymphocyte Reaction (MLR)

 

Check out more supporting materials on antigen-specific T cells