MBTP 35: Immunophenotyping of tumor-infiltrating lymphocytes from human ovarian carcinoma using flow cytometry 

Miltenyi Biotec-tested panel 35 (MBTP 35)

This application protocol describes the characterization of human monocytes, neutrophils, eosinophils, and T, B, and NK lymphocyte populations as well as CD4+, CD8+, and CD56+CD3+ T cell subsets in human ovarian carcinoma using flow cytometry. Prior to staining the tumor was dissociated employing the gentleMACS™ Octo Dissociator with Heaters and Tumor Dissociation Kit, human.

Protocol

Materials

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