Miltenyi Biotec-tested panel 11 (MBTP 11)
This application protocol describes the flow cytometric analysis of major T cell subsets after spleen dissociation from healthy C57BL/6 mice. Mouse spleens can easily be dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD4 | REA604 | T helper cells | VioBlue® | 450/50 (violet) |
Viobility™ 405/520 Fixable Dye | - | Viability | - | 525/50 (violet) |
CD62L | REA828 | Memory T cells | FITC | 525/50 (blue) |
CD127 | REA680 | Infection response | PE | 585/40 (blue) |
CD3e | REA606 | T cells | PE-Vio® 615 | 655-730 (blue) |
CD44 | REA644 | Memory T cells | PE-Vio 770 | 750LP (blue) |
KLRG1 | REA1016 | Infection response | APC | 655-730 (red) |
CD8a | REA601 | Cytotoxic T cells | APC-Vio 770 | 750LP (red) |
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