Miltenyi Biotec-tested panel 11 (MBTP 11)
This application protocol describes the flow cytometric analysis of major T cell subsets after spleen dissociation from healthy C57BL/6 mice. Mouse spleens can easily be dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD4||REA604||T helper cells||VioBlue®||450/50 (violet)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|CD62L||REA828||Memory T cells||FITC||525/50 (blue)|
|CD127||REA680||Infection response||PE||585/40 (blue)|
|CD3e||REA606||T cells||PE-Vio® 615||655-730 (blue)|
|CD44||REA644||Memory T cells||PE-Vio 770||750LP (blue)|
|KLRG1||REA1016||Infection response||APC||655-730 (red)|
|CD8a||REA601||Cytotoxic T cells||APC-Vio 770||750LP (red)|
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