Miltenyi Biotec-tested panel 26 (MBTP 26)
This application protocol describes the simultaneous quantification of intracellular and surface markers for easy enumeration of regulatory T cells from human PBMCs using flow cytometry.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD4||REA623||T helper cells||VioGreen™||525/50 (violet)|
|CD25||REA570||Regulatory T cells||Vio® Bright B515||525/50 (blue)|
|CD127||REA614||Regulatory T cells||PE||585/40 (blue)|
|FoxP3||REA944||Transcription factor of regulatory T cells||Vio 667||655-730 (blue)|
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