Miltenyi Biotec-tested panel 34 (MBTP 34)
This application protocol describes the characterization of CAR T cell persistence and differentiation using flow cytometry. For this, CAR T cells were spiked into whole blood. The data was analyzed using the automated data acquisition and analysis tool Express Mode “CAR_T_Cell_Persistence_h_01”.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
7-AAD Staining Solution | - | Viability | - | 655–730 (blue) |
CD3 | REA613 | T cells | FITC | 525/50 (blue) |
CD4 | REA623 | T helper cells | VioGreen™ | 525/50 (violet) |
CD8 | REA734 | Cytotoxic T cells | APC-Vio® 770 | 750 LP (red) |
CD45RO | REA611 | T cell differentiation | APC | 655-730 (red) |
CD62L | 145/15 | T cell differentiation | PE-Vio 770 | 750 LP (blue) |
CD45RA | REA562 | T cell differentiation | VioBlue® | 450/50 (violet) |
Biotin | REA746 | CAR T cells | PE | 585/40 (blue) |
CD19 CAR Detection Reagent | - | CAR T cells | - | - |
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