Miltenyi Biotec-tested panel 27 (MBTP 27)
This application protocol describes the flow cytometric analysis of B cell subsets after isolation of B cells from human buffy coat. B cells were isolated from human buffy coat using the Pan B Cell Isolation Kit, human to quickly obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|7-AAD Staining Solution||-||Viability||-||655-730 (blue)|
|CD20||REA780||B cells||APC-Vio® 770||750LP (red)|
|IgD||REA740||Naive B cells||VioBlue®||450/50 (violet)|
|CD27||REA499||Memory B cells||PE-Vio 770||750LP (blue)|
|CD38||REA572||Plasmablasts/plasma cells||Vio Bright FITC||525/50 (blue)|
|CD138||REA929||Plasma cells||APC||655-730 (red)|
|CD86||REA968||Activation marker||PE||585/40 (blue)|
|HLA-DR||REA805||Activation marker||VioGreen™||525/50 (violet)|
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