Application protocol

Engineering of CAR NK cells
 for research use

Depletion of donor T cells, enrichment of donor NK cells, in vitro activation, transduction, expansion, phenotyping, and functional analysis.

CAR NK cells have to be developed, optimized, and validated in a pre-clinical setting by small-scale benchtop experiments. This application protocol describes a complete workflow for the engineering of CAR NK cells for research. It includes depletion of donor T cells, enrichment of donor NK cells, followed by activation, gene transfer of the CAR construct, and CAR NK cell expansion as well as the phenotyping and analysis of the final CAR NK cell product.  

Protocol

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This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.

Materials

The following is a listing of reagents, instruments, and consumables needed for each step of this protocol. These products are for research use only.
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