Clone:
REA476
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
EMMPRIN, Basigin (BSG), Neurothelin, 5F7, BSG, TCSF, OKa, CD147

Extended validation for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of TRA-1-85 (CD147) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TRA-1-85 (CD147)-PE (REA476, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of TRA-1-85 (CD147) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TRA-1-85 (CD147)-PE (REA476, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of TRA-1-85 (CD147) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TRA-1-85 (CD147)-PE (REA476, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD147 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD147-PE, clone (REA476). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD147 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD147-PE, clone (REA476). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Overview

Clone REA476 recognizes the human TRA-1-85 antigen, a single-pass type I membrane protein, which is also known as CD147, basigin, or extracellular matrix metalloproteinase inducer (EMMPRIN). TRA-1-85 is expressed by many cell types, including epithelial cells, endothelial cells, leukocytes, and tumor cells. It is present in vascular endothelium in non-neoplastic regions of the brain, and in tumor cells but not in proliferating blood vessels in malignant gliomas. TRA-1-85 has been implicated in a variety of physiological and pathological activities. It is best known for its ability to function as extracellular matrix metalloproteinase inducer, but has also been shown to regulate lymphocyte responsiveness, monocarboxylate transporter expression, and spermatogenesis. TRA-1-85 is often used to discern human cells from non-human cells in multiple applications including animal transplantation studies.
Additional information: Clone REA476 displays negligible binding to Fc receptors.

Alternative names

EMMPRIN, Basigin (BSG), Neurothelin, 5F7, BSG, TCSF, OKa, CD147

Detailed product information

Technical specifications

CloneREA476
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTRA-1-85 (CD147)
Alternative names of antigenEMMPRIN, Basigin (BSG), Neurothelin, 5F7, BSG, TCSF, OKa, CD147
Molecular mass of antigen [kDa]40
Distribution of antigenleukocytes, endothelial cells, epithelial cells, tumor cells
Entrez Gene ID682
RRIDAB_2654232, AB_2654233, AB_2654236, AB_2654237, AB_2654238, AB_2654239, AB_2654240, AB_2654241, AB_2654242, AB_2654243

Resources for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Publications

  1. Biswas, C. et al. (1995) The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobulin superfamily. Cancer Res. 55(2): 434-439
  2. Williams, B. P. et al. (1988) Biochemical and genetic analysis of the OKa blood group antigen. Immunogenetics 27(5): 322-329
  3. Yurchenko, V. et al. (2006) Dealing with the family: CD147 interactions with cyclophilins. Immunology 117(3): 301-309

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