Clone:
REA915
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
TCR Vbeta17, TCR Vb17

Extended validation for TCR Vβ17 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA915
E17.5F3.15.13++
Cells were incubated with an excess of purified unconjugated TCR Vβ17 (REA915) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for TCR Vβ17. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ17 antibodies and with a suitable counterstaining. As a control, TCR Vβ17 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vβ17. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ17 antibodies and with a suitable counterstaining. As a control, TCR Vβ17 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vβ17. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ17 antibodies and with a suitable counterstaining. As a control, TCR Vβ17 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for TCR Vβ17. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ17 antibodies and with a suitable counterstaining. As a control, TCR Vβ17 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ17 (REA915). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ17 (REA915). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ17 (REA915). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vβ17 Antibody, anti-human, REAfinity™

Overview

Clone REA915 recognizes the human variable β 17 subunit of the αβ T cell receptor (TCR Vβ17). The TCR is a heterodimeric glycoprotein associated with the CD3 antigen. The α and β TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. TCR Vβ17 is a variant of the TCR β chain. αβ T cells express a diverse αβ TCR repertoire that specifically co-recognizes self or foreign antigen bound to antigen-presenting molecules, which thereby leads to T cell–mediated immunity. For example, the TCR can directly bind to peptide fragments, riboflavin precursors, and lipid antigens that are presented by major histocompatibility complex (MHC) molecules, MR1 and CD1, respectively. In each case, the antigen sits within the antigen-binding cleft, whereupon the TCR recognizes a composite surface formed by the antigen-presenting molecule and surface-exposed regions of the antigen itself. This co-recognition paradigm is a central tenet of αβ T cell–mediated immunity and underpins MHC restriction.
Additional information: Clone REA915 displays negligible binding to Fc receptors.

Alternative names

TCR Vbeta17, TCR Vb17

Detailed product information

Technical specifications

CloneREA915
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTCR Vβ17
Alternative names of antigenTCR Vbeta17, TCR Vb17
Molecular mass of antigen [kDa]11
Distribution of antigenT cells
Entrez Gene ID28570
RRIDAB_2726958, AB_2727016, AB_2726959, AB_2727017, AB_2726960, AB_2727018, AB_2726961, AB_2727019, AB_2726962, AB_2727014, AB_2726957, AB_2857610, AB_2857597, AB_2727015

Resources for TCR Vβ17 Antibody, anti-human, REAfinity™

Certificates

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References for TCR Vβ17 Antibody, anti-human, REAfinity™

Publications

  1. Kimuram, N. et al. (1987) Sequences and repertoire of the human T cell receptor alpha and beta chain variable region genes in thymocytes. Eur. J. Immunol. 17(3): 375-383
  2. Wei, S. et al. (1994) The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics 40(1): 27-36

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