The StraightFrom
®
Leukopak
®
MicroBead Kit was developed for the rapid positive selection of CD4
+
T cells directly from Leukopak
®
using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Blocks and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak
®

Data and images for
StraightFrom
®
Leukopak
®
CD4 MicroBead Kit
, human

Figures

Figure 1

Separation of a Leukopak
®
sample using the StraightFrom
®
Leukopak
®
CD4 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Blocks. Cells were fluorescently stained with CD4-PE, CD14‑APC, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD4
+
Tʜ cells
View details

Figure 1

Separation of a Leukopak
®
sample using the StraightFrom
®
Leukopak
®
CD4 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Blocks. Cells were fluorescently stained with CD4-PE, CD14‑APC, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of a Leukopak
®
sample using the StraightFrom
®
Leukopak
®
CD4 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Blocks. Cells were fluorescently stained with CD4-PE, CD14‑APC, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
StraightFrom
®
Leukopak
®
CD4 MicroBead Kit
, human

Overview

The StraightFrom
®
Leukopak
®
MicroBead Kit was developed for the rapid positive selection of CD4
+
T cells directly from Leukopak
®
using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Blocks and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak
®
.

Detailed product information

Background information

The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4
+
T helper cells.

Detailed separation procedure

A Leukopak
®
sample is labeled with StraightFrom
®
Leukopak
®
CD4 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus. After removing the Column Block from the magnetic field, the magnetically retained CD4
+
T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD4
+
T cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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StraightFrom
®
Leukopak
®
CD4 MicroBead Kit
, human

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