StemMACS™ Sox2 mRNA encodes the human transcription factor Sox2 for transient transfection.

Data and images for StemMACS™ Sox2 mRNA, human

Figures

Figure 1

Sox2 expression after mRNA transfection:
Human BJ Fibroblasts were transfected once with 150 ng StemMACS™ Sox2 mRNA. 24 hours after transfection, Sox2 expression (magenta) and nuclear localization (colocalization with DAPI) were analyzed by immunofluorescence microscopy.
Untransfected cells
Sox2-transfected cells
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Figure 1

Sox2 expression after mRNA transfection:
Human BJ Fibroblasts were transfected once with 150 ng StemMACS™ Sox2 mRNA. 24 hours after transfection, Sox2 expression (magenta) and nuclear localization (colocalization with DAPI) were analyzed by immunofluorescence microscopy.
View details

Figure 1

Sox2 expression after mRNA transfection:
Human BJ Fibroblasts were transfected once with 150 ng StemMACS™ Sox2 mRNA. 24 hours after transfection, Sox2 expression (magenta) and nuclear localization (colocalization with DAPI) were analyzed by immunofluorescence microscopy.

Specifications for StemMACS™ Sox2 mRNA, human

Overview

StemMACS™ Sox2 mRNA encodes the human transcription factor Sox2 for transient transfection.

Applications

  • Modulation of pluripotency
  • Reprogramming

Detailed product information

Background information

Sox2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Exogenous expression of Sox2 in combination with other transcription factors has been used to generate induced pluripotent stem cells.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

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