StemMACS PSC-Brew XF, human is a new generation culturing medium for highly efficient maintenance and expansion of human pluripotent stem cells (hPSC).

Data and images for StemMACS™ PSC-Brew XF, human

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Figure 1

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Pluripotent stem cells cultured in StemMACS PSC-Brew XF display typical colony morphology (A) and high expression of pluripotency markers (B).

Figure 1

Pluripotent stem cells cultured in StemMACS PSC-Brew XF display typical colony morphology (A) and high expression of pluripotency markers (B).

Figure 2

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Pluripotent stem cells cultured in StemMACS PSC-Brew XF maintain full pluripotent differentiation potential and can differentiate in all three embryonic germ layers.

Figure 2

Pluripotent stem cells cultured in StemMACS PSC-Brew XF maintain full pluripotent differentiation potential and can differentiate in all three embryonic germ layers.

Figure 3

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Pluripotent stem cells cultured in StemMACS PSC-Brew XF maintain a normal genotype over passages as shown from karyotype analysis (A) and copy number variants (CNV) analysis (B).

Figure 3

Pluripotent stem cells cultured in StemMACS PSC-Brew XF maintain a normal genotype over passages as shown from karyotype analysis (A) and copy number variants (CNV) analysis (B).

Specifications for StemMACS™ PSC-Brew XF, human

Overview

StemMACS PSC-Brew XF, human is a new generation culturing medium for highly efficient maintenance and expansion of human pluripotent stem cells (hPSC).

Detailed product information

Background information

StemMACS PSC-Brew XF, human is a new generation cell culture medium for highly efficient maintenance and expansion of human pluripotent stem cells (hPSC). It has a xeno-free and serum-free formulation that ensures a reduced variability and standardized performances during culture. The carefully balanced composition and the presence of stable FGF-2 ensures constant exposure levels and provides flexibility with feeding schedules.
StemMACS PSC-Brew XF, human is compatible with both defined and undefined cell attachment matrices and allows the maintenance of a highly consistent pluripotent phenotype even in low density cultures. hPSCs grown in StemMACS PSC-Brew XF, human show a typical morphology, expression of pluripotency-associated markers and retain the ability to differentiate into cell types that derive from the three embryonic germ layers. Moreover, the maintenance of a stable karyotype in long-term cultivation is supported.
StemMACS PSC-Brew XF, human allows rapid culture re-initiation of hPSC cultures after cryopreservation and enables cluster passaging without the addition of any inhibitor.
StemMACS PSC-Brew XF, human is fully compatible with StemMACS PSC-Support XF, human and, when combined, can be used in challenging applications that would normally stress the cells, such as gene editing approaches or after reprogramming.

Applications

Highly efficient maintenance and expansion of hPSCs under xeno- and feeder-free conditions.

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