The Pluripotent Stem Cell Isolation Kit has been specifically designed for the depletion of early differentiated cells from mouse ES and iPS cell cultures. Based on a novel marker that discriminates between pluripotent and early differentiated cells, the kit guarantees the fast and easy isolation of homogeneous ES and iPS cell populations.

Data and images for Pluripotent Stem Cell Isolation Kit, mouse

Figures

Figure 1

Mouse ES cell cultures containing 14% early differentiated cells (A), as they can occur during feeder-free cultivation, were separated using the Pluripotent Stem Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Differentiated cells were completely removed resulting in a purity of approx. 99% pluripotent cells (B). Flow cytometric analysis using the MACSQuant® Analyzer. Debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.
A:
B:
Unseparated fraction
After separation
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Figure 1

Mouse ES cell cultures containing 14% early differentiated cells (A), as they can occur during feeder-free cultivation, were separated using the Pluripotent Stem Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Differentiated cells were completely removed resulting in a purity of approx. 99% pluripotent cells (B). Flow cytometric analysis using the MACSQuant® Analyzer. Debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse ES cell cultures containing 14% early differentiated cells (A), as they can occur during feeder-free cultivation, were separated using the Pluripotent Stem Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Differentiated cells were completely removed resulting in a purity of approx. 99% pluripotent cells (B). Flow cytometric analysis using the MACSQuant® Analyzer. Debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.

Figure 2

Phase contrast pictures of fixed feeder-free mouse embryonic stem cell cultures one day after plating without (A) and with separation (B) indicate efficient removal of differentiated cells.
A:
B:
Without separation
With separation
View details

Figure 2

Phase contrast pictures of fixed feeder-free mouse embryonic stem cell cultures one day after plating without (A) and with separation (B) indicate efficient removal of differentiated cells.
View details

Figure 2

Phase contrast pictures of fixed feeder-free mouse embryonic stem cell cultures one day after plating without (A) and with separation (B) indicate efficient removal of differentiated cells.

Specifications for Pluripotent Stem Cell Isolation Kit, mouse

Overview

The Pluripotent Stem Cell Isolation Kit has been specifically designed for the depletion of early differentiated cells from mouse ES and iPS cell cultures. Based on a novel marker that discriminates between pluripotent and early differentiated cells, the kit guarantees the fast and easy isolation of homogeneous ES and iPS cell populations.

Detailed product information

Background information

Expression of markers, such as the transcription factors Oct-4 and Nanog or the surface carbohydrate SSEA-1 (CD15), are mainly used to characterize pluripotency of mouse cells on a molecular level. Nevertheless, expression dynamics of these markers are rather low, limiting their potential to discriminate between pluripotent and early differentiated cells. Our scientists have identified a cell surface marker that is strongly up-regulated during the early differentiation of mouse ES and iPS cells in culture. The novel Pluripotent Stem Cell Isolation Kit, mouse, was established on this basis and allows to easily deplete the early differentiated cells resulting in homogeneous pluripotent stem cell cultures.

Downstream applications

Depletion of early differentiated cells enables:
  • standardized pluripotent stem cell culture
  • standardized pluripotent stem cell differentiation experiments
  • profiling of pluripotent stem cells without contamination of differentiated cells
  • efficient generation of transgenic mice using pluripotent stem cells

Columns

LD or autoMACS
®
Columns.

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