The Neutrophil Isolation Kit has been developed for the isolation of untouched neutrophils from mouse bone marrow or blood cell suspensions.

Data and images for Neutrophil Isolation Kit, mouse

Figures

Figure 1

Neutrophils were isolated from C57BL/6 bone marrow cells using the Neutrophil Isolation Kit, mouse, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Anti-Ly-6G-PE and CD11b-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
Unseparated fraction
Isolated neutrophils
View details

Figure 1

Neutrophils were isolated from C57BL/6 bone marrow cells using the Neutrophil Isolation Kit, mouse, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Anti-Ly-6G-PE and CD11b-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
View details

Figure 1

Neutrophils were isolated from C57BL/6 bone marrow cells using the Neutrophil Isolation Kit, mouse, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Anti-Ly-6G-PE and CD11b-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

Specifications for Neutrophil Isolation Kit, mouse

Overview

The Neutrophil Isolation Kit has been developed for the isolation of untouched neutrophils from mouse bone marrow or blood cell suspensions.

Detailed product information

Background information

Neutrophils are essential for host defense against invading pathogens. They are phagocytic polymorphonuclear cells, which engulf and degrade microorganisms using a wide range of toxic agents including reactive oxygen species, antimicrobial peptides, and proteases. Besides, neutrophils can produce anti-inflammatory molecules and factors that promote the resolution of inflammation.

Detailed separation procedure

Non-neutrophils are incubated with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion.

Downstream applications

  • Functional studies on neutrophils.
  • Studies on neutrophil activation, differentiation, cytokine secretion etc.
  • Studies on signal transduction in neutrophils.

Columns

LS Columns for neutrophil isolation from bone marrow, MS Columns for neutrophil isolation from peripheral blood, or autoMACS
®
Columns.

Resources for Neutrophil Isolation Kit, mouse

Documents and Protocols

Reviews for Neutrophil Isolation Kit, mouse

Easy Way to Isolate Polymorphonuclear Cells from Bone Marrow

  • 1
  • 2
  • 3
  • 4
  • 5

Neutrophil Isolation Kit, mouse (130-097-658)

Isolated PMN directly from murine bone marrow, great results (easier and faster than FACS, excellent results).

Seems like you are coming from USA!
Do you want to visit our website in your country?