LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Mouse LIF

Figures

Figure 1

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Mouse LIF activity assay.
The biological activity of Mouse LIF was determined by inhibition assay using mouse M1 cells. Activity of Mouse LIF, premium grade (red line) was compared to another commercially available product (black line) with fully equivalent results.

Figure 1

Mouse LIF activity assay.
The biological activity of Mouse LIF was determined by inhibition assay using mouse M1 cells. Activity of Mouse LIF, premium grade (red line) was compared to another commercially available product (black line) with fully equivalent results.

Figure 2

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SDS-PAGE of Mouse LIF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Mouse LIF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Mouse LIF, premium grade. The peak corresponds to the calculated molecular mass of 19862 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Mouse LIF, premium grade. The peak corresponds to the calculated molecular mass of 19862 Da.

Specifications for Mouse LIF

Overview

LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse LIF can be used for a variety of applications including:
  • Maintenance of self-renewal and pluripotency in conventional mouse ESC cultures.

Detailed product information

Background information

LIF is a pleiotropic cytokine, which is critically involved in embryonic development and blastocyst implantation. LIF belongs to the interleukin 6 family and functions through the gp130 activation of STAT3. In mice LIF is a key factor that prevents embryonic stem cells (ESC) to differentiate. Additionally, LIF affects hematopoiesis, neural development, bone and energy metabolism, and inflammation.

Biological activity

  • Inhibition of M1 cells
  • premium grade: ≥ 2×
    10
    6
    U/mg
    (typical activity: ≥ 2.5×
    10
    6
    U/mg
    )
  • research grade: ≥ 1×
    10
    6
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

References for Mouse LIF

Publications

  1. Rose, T.M. and Bruce, A.G. (1991) Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc. Natl. Acad. Sci. U.S.A. 88: 8641-8645
  2. Barral, S. et al. (2013)
    Efficient neuronal
    in vitro
    and
    in vivo
    differentiation after immunomagnetic purification of mESC derived neuronal precursors.
    Stem Cell Res. 10(2): 133-46

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