IL-2 IS stands for interleukin 2 “improved sequence” and is a variant of IL-2 with the serine substituted for the native cysteine at amino acid position 160.
Mouse IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Mouse IL-2 IS

Figures

Figure 1

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Mouse IL-2 IS activity assay.
The biological activity of Mouse IL-2 IS, premium grade was determined by proliferation assay using CTLL-2 cells.

Figure 1

Mouse IL-2 IS activity assay.
The biological activity of Mouse IL-2 IS, premium grade was determined by proliferation assay using CTLL-2 cells.

Figure 2

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SDS-PAGE of Mouse IL-2 IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Mouse IL-2 IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Mouse IL-2 IS biological activity.
Activity of Mouse IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Mouse IL-2 IS biological activity.
Activity of Mouse IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

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Mass spectrometry analysis (ESI-MS) of Mouse IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 17213 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Mouse IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 17213 Da.

Specifications for Mouse IL-2 IS

Overview

IL-2 IS stands for interleukin 2 “improved sequence” and is a variant of IL-2 with the serine substituted for the native cysteine at amino acid position 160.
Mouse IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-2 IS can be used for a variety of applications, including:
  • In vitro activation and propagation of mouse T cells, e.g., using the T Cell Activation/Expansion Kit, mouse.
  • In vitro activation and expansion of NK cells.
  • Generation of lymphokine-activated killer (LAK) cells.

Detailed product information

Background information

Interleukin 2 (IL-2), a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. IL-2 is produced by activated T cells, especially the CD4
+
T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells, and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- and common γ-chain. The later two are also used for IL-15 signaling.

Biological activity

  • Proliferation of mouse CTLL-2 cells
  • premium grade: ≥ 2×
    10
    7
    U/mg
    (typical activity: ≥ 2×
    10
    7
    U/mg
    )
  • research grade: ≥ 8×
    10
    6
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse IL-2 IS

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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